一株芒果蒂腐病病原菌的鉴定及中草药对其抑制作用分析  

Identification of Pathogen Causing Stem End Rot of Mango and Analysis of Inhibitory Effect by Chinese Herbal Medicine

作　　者：欧阳秋飞[1] 杨建波 马博[1] 陆家明 骆建宇 OUYANG Qiu-fei;YANG Jian-bo;MA Bo;LU Jia-ming;LUO Jian-yu(Baise University,Baise 533000,China)

机构地区：[1]百色学院

出　　处：《江西农业学报》2019年第12期54-59,共6页Acta Agriculturae Jiangxi

基　　金：广西高校中青年教师基础能力提升项目(KY2016YB415、KY2016YB416);广西自然科学基金青年科学基金项目(2017JJ B130048);广西高等学校优势特色专业群建设项目[桂教高教(2015)41号];百色学院特色研究团队建设项目[百院字(2015)148号];广西本科高校特色专业及实验实训教学基地(中心)建设项目“设施农业科学与工程”

摘　　要：从百色右江河谷采后芒果病样上分离、纯化得到多个真菌菌株,选择1个典型分离菌BSD01进行致病性测定、形态学观察、ITS序列测定和同源性分析。同时采用生长速率法,测定15种中草药提取液对该病原菌的抑制作用,确定了有效提取液的种类及其最低抑菌浓度(MIC)。结果表明,能引起右江河谷芒果蒂腐病的病原菌为可可球二孢菌(Botryodiplodia theobromae Pat.)。在供试的15种中草药提取液中,醇提取液的抑菌活性总体高于水提取液。其中,在0.06 g/mL的剂量下,莱菔子水提取液和益母草醇提取液的抑菌效果最好,抑菌率可达到100%;其次为石菖蒲和莱菔子醇提取液,抑菌率分别为94.59%和73.21%。它们对芒果蒂腐病菌的MIC值分别为<0.01、0.02、0.11、0.09 g/mL。Several fungal strains were separated and purified from the diseased Mango samples which collected from the river valley of Youjiang in Baise City.A typical isolate BSD01 was selected for pathogenicity determination,morphological observation,ITS sequence determination and homology analysis.By the mycelium growth rate method,the inhibitory effect of 15 kinds of Chinese herb extracts by water or alcohol on the pathogenic fungi were investigated respectively.The types of effective extracts and their minimum inhibitory concentration(MIC)were determined.The results showed that the pathogenic fungus was Botryodiplodia theobromae Pat.Among these 15 Chinese herbal extracts,the inhibitory effect of the alcohol extract was higher than that water extract.Among which,the inhibitory effect of 0.06 g/mL water extract of radish seed and 0.06 g/mL alcohol extract of Herba leonuri were best,resulting in a rate of 100%,followed by alcohol extract of Acorus tatarinowii and radish seed.The inhibition rate was 94.59%and 73.21%respectively.The minimum inhibitory concentration(MIC)values of the above four Chinese herb extracts were<0.01 g/mL,0.02 g/mL,0.11 g/mL and 0.09 g/mL respectively.

关 键 词：芒果 蒂腐病菌 中草药 抑菌 

分 类 号：S436.6[农业科学—农业昆虫与害虫防治]