玉米蔗糖合成酶基因家族的全基因组鉴定及表达分析  

Genome-wide Identification and Expression Analysis of Sucrose Synthase Family in Maize

作　　者：陈曙[1,2] 赵秋芳 陈宏良[1,2] 金辉 CHEN Shu;ZHAO Qiu-fang;CHEN Hong-liang;JIN Hui(Institute of South Subtropical Corp Research,Chinese Academy of Tropical Agricultural Science,Guangdong Zhanjiang 524091,China;Key Laboratory of Tropic Fruit Biology,Ministry of Agriculture,Guangdong Zhanjiang 524091,China)

机构地区：[1]中国热带农业科学院南亚热带作物研究所,广东湛江524091 [2]农业部热带果树生物学重点实验室,广东湛江524091

出　　处：《西南农业学报》2019年第11期2479-2485,共7页Southwest China Journal of Agricultural Sciences

基　　金：中央级非盈利性科研院所基本科研业务“糯玉米种质资源的收集、评价及自交系的选育”(1630062017015);农业部国际交流与合作项目“‘一带一路’沿线国家热带农业资源联合调查与开发评价”(13232697606221573):热带玉米种质资源联合调查与评价

摘　　要：【目的】蔗糖合成酶(SUS)是植物进行蔗糖代谢的关键酶之一,控制着植物体内糖分的合成和运输,在植物的生长发育和代谢活动中具有重要作用。【方法】本研究利用玉米基因组数据库平台,以生物信息学手段对玉米SUS基因家族进行成员鉴定和分析。【结果】①玉米SUS家族共有5个成员,它们分别分布在第1.4.9号染色体上。氨基酸大小为802~849 aa,除ZmSUS5为弱碱性以外,其余均表现为弱酸性。②基因二级结构分析显示,该家族蛋白主要结构为a-螺旋,大部分为不稳定蛋白,且具有弱疏水性。③亚细胞定位预测结果显示,ZmSUS蛋白均定位在叶绿体。④进化分析显示ZmSUS可分为3个亚家族(SUSⅠ~SUSⅢ),且位于同一组的成员其氨基酸序列一致性以及内含子/外显子分布模式相似度都很高。⑤基因启动子分析分析显示ZmSUS家族蛋白在玉米的生长发育以及逆境胁迫方面都起着重要的作用。⑥组织表达特异性分析发现,ZmSUS1和ZmSUS2在胚和胚乳中大量表达;ZmSUS5在所有被检测的组织中表达量很低;ZmSUS3及ZmSUS4在被检测的组织中均有较高的表达量。【结论】这些结果表明ZmSUS1和ZmSUS2可能参与了种子的发育过程,ZmSUS3、ZmSUS4可能参与玉米生长发育的多个阶段。本研究为后续对玉米SUS家族基因的功能研究以及对蔗糖代谢调控的应用提供了理论参考。【Objective】Sucrose Synthases are involved in many aspects of plant activities,such as plant grow,breeding and metabolish,which is the pivotal enzyme of plant sucrose reaction.【Method】In this study,we took advantage of bioinformatics to identify and analyze maize SUS gene family members based on corn genome database.【Result】Our results showed as follows:(i)The maize SUS gene family contained five members,unevenly located on chromosome 1,4,9.Amino acids length range from 802 aa to 849 aa.Only ZmSUS5 display weak alkalinity,the other members all show feeble acidity.(ii)Alpha helix was confirmed as the primary structure of the SUS proteins in maize.Besides,most proteins revealed unstable and hydrophobic characteristic.(iii)Subcellular localization prediction showed all maize SUS proteins locate on chloroplast.(iv)A Phylogenetic analysis indicated that SUS genes in maize could be divided into three subfamilies(SUSⅠ-SUS Ⅲ),while genes consist in the same subfamily had similar encoded amino acid sequence.(v)Gene promoter analysis showed SUS proteins in maize had some motifs related toplant growth,breeding and stress defense,which meant the SUS genes may played a role about this activities.(vi)Moreover,expression pattern analysis indicated ZmSUS1 and ZmSUS2 expressed abundant in embryo and endosperm.ZmSUS5 showed little expression in all the inspected tissues;oppositely,ZmSUS3 and ZmSUS4 were found expressed in all the tissues.【Conclusion】The results implied that ZmSUS1 and ZmSUS2 were involved in the activities about seed metabolism.Dissimilarly,ZmSUS3 and ZmSUS4 were detected expressed highly in multiple phases through the whole maize growth period,which means the two genes may be closely related to mazie growth and development.This research may lay a foundation for the future study of SUS gene function and improvement of the utilization of sucrose metabolism.

关 键 词：玉米 SUS基因家族 生物信息学分析 系统进化分析 表达分析 

分 类 号：S513[农业科学—作物学]