长非编码RNA PANDAR在舌鳞状细胞癌中的功能研究  

The role of lncRNA PANDAR in tongue squamous cell carcinoma

作　　者：方松城[1] 修霞 麦潋曦 谢舒乐 林钊宇[3] 焦九阳[3] FANG Songcheng;XIU Xia;MAI Lianxi;XIE Shule;LIN Zhaoyu;JIAO Jiuyang(Department of Stomatology,Donghua hospital,Dongguan 523110,China;Department of Stomatol⁃ogy,Huangdao District Central Hospital,Qingdao 266000,China;Department of Stomatology,Sun Yet⁃san Memorial Hospital,Guangzhou 510120,China)

机构地区：[1]东莞东华医院口腔科,广东东莞523110 [2]青岛市黄岛区中心医院,山东青岛266000 [3]中山大学孙逸仙纪念医院口腔科,广州510120

出　　处：《岭南现代临床外科》2019年第6期701-705,共5页Lingnan Modern Clinics in Surgery

基　　金：国家自然科学基金青年项目（81602379,81903045）;中国博士后科学基金（2018M643352）

摘　　要：目的探究长非编码RNA(lncRNA)PANDAR在舌鳞状细胞癌中的表达及调控机制。方法利用实时定量荧光PCR(q RT-PCR)检测PANDAR在舌鳞癌临床样本及细胞系的表达水平,通过CCK-8法及流式细胞仪分析检测敲低PANDAR对舌癌细胞增殖和凋亡的影响。qRT-PCR和Western-blot检测敲低PANDAR对凋亡相关基因Bax表达的影响。结果 LncRNA PANDAR在舌鳞癌样本和细胞系内均显著高表达。敲低PANDAR可抑制Cal27和SCC25细胞的增殖并促进其凋亡。此外,敲低PANDAR有利于Bax蛋白的表达。结论 PANDAR在舌鳞癌组织中高表达。PANDAR通过下调促凋亡基因Bax的表达,促进舌鳞癌细胞的增殖并抑制其凋亡。Objective To investigate the expression of lncRNA PANDAR in tongue squamous cell carcinoma(TSCC) and explore its function and mechanism.Methods The expression of PANDAR was determined by qRT-PCR in tongue squamous cell carcinoma specimens and cell lines.The CCK8 assays and flow cytometry were used to detect cell proliferation and apoptosis of tongue squamous cell carcinoma cells with or without PANDAR deletion.The expression of Bax in Cal27 and SCC25 cells was detected by qRT-PCR and Western blot.Results LncRNA PANDAR-was significantly upregulated in TSCC tissues and cell lines.PANDAR knockdown improved cell proliferation,but suppressed cell apoptosis.PANDAR deletion was beneficial to the expression of Bax in Cal27 and SCC25 cells.Conclusion PANDAR is highly expressed in TSCC tissues and cells.PANDAR downregulation in TSCC cells promotes cell proliferation,but inhibits cell apoptosis through inhibiting Bax expression.

关 键 词：长非编码RNA PANDAR BAX 舌鳞状细胞癌 

分 类 号：R739.86[医药卫生—肿瘤]