The Effect of Organic Carbon on Soil Bacterial Diversity in an Antarctic Lake Region  

作　　者：HAN Wenbing WANG Nengfei MA Yue LV Jinjiang WANG Shuang ZHANG Botao JIANG Zhihui CAO Huansheng 

机构地区：[1]College of Chemistry and Chemical Engineering,Qingdao University,Qingdao 266071,China [2]Key Laboratory of Marine Bioactive Substances,First Institute of Oceanography,State Oceanic Administration,Qingdao 266061,China [3]Department of Bioengineering,College of Marine Sciences and Biological Engineering,Qingdao University of Science&Technology,Qingdao 266042,China [4]School of Basic Medicine,Qingdao University,Qingdao 266071,China [5]Key Laboratory of Marine Science and Numerical Modeling,State Oceanic Administration,Qingdao 266061,China [6]Center for Fundamental and Applied Microbiomics,Biodesign Institute,Arizona State University,Tempe,AZ 85287,USA

出　　处：《Journal of Ocean University of China》2019年第6期1402-1410,共9页中国海洋大学学报（英文版）

基　　金：supported by the National Natural Science Foundation of China (No. 41776198);the Basic Scientific Fund for National Public Research Institutes of China (No. GY0219Q10);the Development Fund of Marine Bioactive Substances, SOA (No. MBSMAT-201701)

摘　　要：This study assessed the effects of changes in organic carbon content on soil bacterial community composition and diversity in the Antarctic Fildes Peninsula.16 S rRNA gene sequencing was performed to investigate bacterial community composition.Firstly,we found that organic carbon(OrC)and nutrients showed an increasing trend in the lake area.Secondly,soil geochemistry changes affected microbial composition in the soil.Specifically,we found 3416 operational taxonomical units(OTUs)in 300 genera in five main phyla:Proteobacteria,Actinobacteria,Acidobacteria,Chloroflexi,and Bacteroidetes.Although the diversity was similar among the four sites,the composition was different.Among them,Hungateii content changed very significantly,from 16.67% to 33.33%.Canonical correspondence analysis showed that most measured geochemical factors were relevant in structuring microbiomes,and organic carbon concentration showed the highest correlation,followed by NO3^--N.Hungateii was significantly correlated with the content of organic carbon.Our finding suggested organic carbon played an important role in soil bacterial communities of the Antarctic coastal lake region.This study assessed the effects of changes in organic carbon content on soil bacterial community composition and diversity in the Antarctic Fildes Peninsula. 16 S rRNA gene sequencing was performed to investigate bacterial community composition. Firstly, we found that organic carbon（OrC） and nutrients showed an increasing trend in the lake area. Secondly, soil geochemistry changes affected microbial composition in the soil. Specifically, we found 3416 operational taxonomical units（OTUs） in 300 genera in five main phyla: Proteobacteria, Actinobacteria, Acidobacteria, Chloroflexi, and Bacteroidetes. Although the diversity was similar among the four sites, the composition was different. Among them, Hungateii content changed very significantly, from 16.67% to 33.33%. Canonical correspondence analysis showed that most measured geochemical factors were relevant in structuring microbiomes, and organic carbon concentration showed the highest correlation, followed by NO3--N. Hungateii was significantly correlated with the content of organic carbon. Our finding suggested organic carbon played an important role in soil bacterial communities of the Antarctic coastal lake region.

关 键 词：BACTERIAL community composition GEOCHEMICAL factor HIGH-THROUGHPUT SEQUENCING organic carbon 

分 类 号：P59[天文地球—地球化学]