Characterization and Functional Study on Octopus ocellatus Interleukin-17  

作　　者：LI Zan FAN Tingting LIU Xintian LIU Xiumei WANG Weijun WANG Qianqian YOU Lunhe WANG Lei WEI Xiumei YANG Jianmin 

机构地区：[1]School of Agriculture,Ludong University,Yantai 264025,China [2]Weihai Fisheries Technology Extension Center,Weihai 264200,China [3]College of Life Sciences,Yantai University,Yantai 264005,China [4]College of Life Sciences,Ludong University,Yantai 264025,China [5]School of Life Sciences,East China Normal University,Shanghai 200241,China

出　　处：《Journal of Ocean University of China》2019年第6期1443-1450,共8页中国海洋大学学报（英文版）

基　　金：supported by the earmarked fund for the Modern Agro-Industry Technology Research System (No. CARS-49);the Natural Science Foundation of Shandong Province (No. ZR2019BC052);the Marine and Fisheries Science and Technology Innovation Program of Shan-dong Province (No. 2017YY04)

摘　　要：Interleukin-17(IL-17),a prototype member of IL-17 family,plays an important role in defending against extracellular pathogens as a pro-inflammatory cytokine.The function and distribution of IL-17 have been extensively studied in many vertebrates.However,few study has focused on the role of IL-17 in invertebrates,especially in mollusks.In this study,an IL-17 homolog was identified in Octopus ocellatus,which was designated as OoIL-17.The phylogenetic analysis showed that OoIL-17 is clustered well with other invertebrate IL-17,indicating it is highly similar with the IL-17 of other invertebrates.The expression of OoIL-17 gene was analyzed with qRT-PCR in a variety of healthy tissues and the hemocytes infected with Vibro anguillarum or Micrococcus luteus.The mRNA of OoIL-17 gene is constitutively expressed at different levels in all examined tissues of healthy O.ocellatus,including mantle,stomach,hemocytes,muscle,gonad,hepatopancreas,systemic heart and gill.The lowest expression was observed in mantle while the highest was observed in hepatopancreas.The expression level of OoIL-17 gene is significantly up-regulated in O.ocellatus hemocytes upon infection with V.anguillarum and M.luteus,indicating its active involvement in the host immune response against bacterial pathogens.The results laid the foundation for further understanding the innate immune mechanisms of IL-17 in O.ocellatus and mollusks.Interleukin-17(IL-17), a prototype member of IL-17 family, plays an important role in defending against extracellular pathogens as a pro-inflammatory cytokine. The function and distribution of IL-17 have been extensively studied in many vertebrates. However, few study has focused on the role of IL-17 in invertebrates, especially in mollusks. In this study, an IL-17 homolog was identified in Octopus ocellatus, which was designated as OoIL-17. The phylogenetic analysis showed that OoIL-17 is clustered well with other invertebrate IL-17, indicating it is highly similar with the IL-17 of other invertebrates. The expression of OoIL-17 gene was analyzed with qRT-PCR in a variety of healthy tissues and the hemocytes infected with Vibro anguillarum or Micrococcus luteus. The mRNA of OoIL-17 gene is constitutively expressed at different levels in all examined tissues of healthy O. ocellatus, including mantle, stomach, hemocytes, muscle, gonad, hepatopancreas, systemic heart and gill. The lowest expression was observed in mantle while the highest was observed in hepatopancreas. The expression level of OoIL-17 gene is significantly up-regulated in O. ocellatus hemocytes upon infection with V. anguillarum and M. luteus, indicating its active involvement in the host immune response against bacterial pathogens. The results laid the foundation for further understanding the innate immune mechanisms of IL-17 in O. ocellatus and mollusks.

关 键 词：IL-17 OCTOPUS OCELLATUS Vibro ANGUILLARUM MICROCOCCUS luteus 

分 类 号：R73[医药卫生—肿瘤]