岩藻黄素纳米复合物的制备及抗氧化、抗肿瘤活性研究  

PREPARATION OF FUCOXANTHIN NANOCOMPLEXS AND THEIR ANTIOXIDANT AND ANTITUMOR ACTIVITY

作　　者：孙潇雯 赵海龙 赵丽丽 徐莹[1] 汪东风[1] SUN Xiao-Wen;ZHAO Hai-Long;ZHAO Li-Li;XU Ying;WANG Dong-Feng(College of Food Science and Technology,Ocean University of China,Qingdao 266003,China;Research Vessel Center,Ocean University of China,Qingdao 266003,China;State Key Laboratory of Bioactive Seaweed Substances,Qingdao Brightmoon Seaweed Group Co Ltd,Qingdao 266400,China)

机构地区：[1]中国海洋大学食品科学与工程学院,青岛266003 [2]中国海洋大学船舶中心,青岛266003 [3]青岛明月海藻集团有限公司海藻活性物质国家重点实验室,青岛266400

出　　处：《海洋与湖沼》2019年第6期1233-1240,共8页Oceanologia Et Limnologia Sinica

基　　金：青岛市黄岛区自主创新重大项目,2014-3-11号;海藻活性物质国家重点实验室开放基金资助项目,SKL-BASS1704号

摘　　要：岩藻黄素是一种脂溶性类胡萝卜素,由于其水溶性差、环境敏感性强,使其应用受到限制。本研究采用有机溶剂浸提、大孔树脂纯化法,从裙带菜中提取高纯度岩藻黄素。通过复凝聚反应制备乳清分离蛋白-岩藻黄素-阿拉伯胶纳米复合物。通过透射电镜观察,纳米复合物呈光滑的球形,粒径约为44nm。红外光谱则表明,岩藻黄素与乳清分离蛋白、阿拉伯树胶发生相互作用,导致联烯键、乙酰基等特征吸收峰被遮蔽。与单一岩藻黄素相比,纳米包封的岩藻黄素具有较好的抗氧化活性稳定性。抗肿瘤实验表明,纳米复合物保留了岩藻黄素抑制HT29细胞增殖的生物活性。Fucoxanthin,a liposoluble carotenoid,has a limited application in food industry due to poor water solubility and strong environmental sensitivity.In the paper,high-purity fucoxanthin was prepared by organic solvent extraction in macroporous resin column chromatography form Undaria pinnatifida;then,fucoxanthin loaded whey protein isolated(WPI)-gum Arabic(GA)nanocomplexes were developed using complex coacervation.The nanocomplexes presented a smooth spherical shape with a diameter of about 44 nm under TEM.The Fourier Transform infrared spectroscopy show that the typical peak of allenic bond and acetyl group vanished,indicating there was interaction between fucoxanthin and WPI/GA.The fucoxanthin nanocomplexes showed a higher antioxidant activity stability than free fucoxanthin.In addition,anti-tumor assay showed that the nanocomplexes retained the biological activity of fucoxanthin to inhibit the proliferation of HT29 cells.

关 键 词：裙带菜 岩藻黄素 岩藻黄素纳米复合物 抗氧化 抗肿瘤 

分 类 号：R284[医药卫生—中药学] R285[医药卫生—中医学]