口腔鳞状细胞癌患者血清单酰甘油酯酶的水平及临床意义  

Serum level and clinical significance of monoglyceride lipase in patients with oral squamous cell carcinoma

作　　者：陈宏丽 柴菲 尹刚 CHEN Hongli;CHAI Fei;YIN Gang(Department of Stomatology,Fenyang College,Shanxi Medical University,Fenyang 032200,China)

机构地区：[1]山西医科大学汾阳学院口腔医学教研室,山西汾阳032200 [2]山西医科大学汾阳学院病理教研室,032000 [3]山西医科大学附属汾阳医院口腔科,032000

出　　处：《临床肿瘤学杂志》2019年第11期1008-1013,共6页Chinese Clinical Oncology

摘　　要：目的探讨口腔鳞状细胞癌(OSCC)患者血清单酰甘油酯酶(MGLL)的水平及临床意义。方法采用基因表达谱动态分析(GEPIA)MGLL在33类肿瘤组织中的表达情况;收集本院2013年1月至2016年12月的初治94例OSCC患者及同期96例健康体检者的血清标本,采用荧光定量PCR(QPCR)检测上述血清的MGLL水平并采用受试者工作特征(ROC)曲线评估血清MGLL水平诊断OSCC的效能,以血清MGLL水平的均数为参考分为低水平(≤均数)和高水平(>均数),进一步分析血清MGLL水平与OSCC临床病理特征和预后的关系,采用Cox比例风险模型分析影响OSCC预后的因素。结果GEPIA在线分析发现在33类肿瘤中,15类肿瘤组织的MGLL表达异常,其中5类呈升高趋势,余10类呈降低趋势,其中头颈部鳞状细胞癌的MGLL水平中位数为19.4,低于正常组织的65.94(P<0.05)。QPCR结果显示,OSCC患者的血清MGLL水平为0.471±0.264,低于健康体检者的1.501±0.687(P<0.05);ROC显示血清MGLL水平诊断OSCC的曲线下面积为0.933(95%CI:0.897~0.968),选择0.903为诊断阈值时约登指数最大(0.7595),灵敏度为95.74%,特异度为80.21%。MGLL水平与年龄和性别无关,而与肿瘤大小、吸烟史、病理分级、淋巴结转移和TNM分期有关(P<0.05)。MGLL低水平的中位总生存期为22.5个月,少于高表达组(未达),差异有统计学意义(P<0.05);多因素分析显示MGLL水平是影响OSCC预后的独立因素(OR=1.355,95%CI:1.115~1.647)。结论OSCC患者的血清MGLL水平为低表达,参与了OSCC的发生发展且与预后不良有关,可作为OSCC诊断和预后评估的潜在指标。Objective To investigate the serum level and clinical significance of monoglyceride lipase(MGLL)in patients with oral squamous cell carcinoma(OSCC).Methods Gene Expression Profiling Interactive Analysis(GEPIA)was used to analyze the expression patterns of MGLL in 33 kinds of cancer tissues.From January 2013 to December 2016,serum samples of 94 patients with OSCC and 96 physical examinees were collected.The quantitative real-time PCR(QPCR)was used to detect the MGLL level in the above serum samples and receiver operating characteristic(ROC)curve was applied to evaluate the efficacy of serum MGLL level in diagnosis of OSCC.According to the mean value of serum MGLL level,the patients were divided into low level(≤mean)and high level(>mean).The relationship between serum MGLL levels and clinicopathological characteristics and prognosis was analyzed.Cox multivariate risk model was used to analyze the prognostic factors of OSCC.Results GEPIA on-line analysis showed that the expression of MGLL was abnormal in 15 kinds of tumors,among which 5 kinds were increased and other 10 kinds were decreased.The median level of MGLL in squamous cell carcinoma of head and neck was 19.4,lower than 65.94 in normal tissues(P<0.05).The results of QPCR showed that the serum MGLL levels of patients with OSCC was 0.471±0.264,lower than 1.501±0.687 of the physical examinees(P<0.05).The area under curve of ROC showed that the serum MGLL level was 0.933(95%CI:0.897-0.968).When selecting 0.903 as the diagnostic threshold,the yoden index was the highest(0.7595)with the sensitivity of 95.74%and the specificity of 80.21%.MGLL level was not related to age and gender,but related to tumor size,smoking history,pathological grade,lymph node metastasis and TNM stage(P<0.05).The median overall survival of patients with low MGLL level was 22.5 months,lower than that of patients with high level(P<0.05).Multivariate analysis showed that MGLL level was an independent factor affecting the prognosis of OSCC(OR=1.355,95%CI:1.115-1.647).Conclusion The serum l

关 键 词：口腔鳞状细胞癌 单酰甘油酯酶 临床意义 预后 

分 类 号：R739.8[医药卫生—肿瘤]