GSK3β对食管癌KYSE450细胞迁移、侵袭和克隆增殖方面调控作用的研究  

Studies on the Regulatory Effects of GSK3β on the Migration,Invasion and Clonal Proliferation of Esophageal Cancer KYSE450 Cells

作　　者：原翔 刘怡文 孙蔚 孔金玉 张灏 高社干 YUAN Xiang;LIU Yi-wen;SUN Wei;KONG Jin-yu;ZHANG Hao;GAO She-gan(College of Clinical Medicine,the First affiliated Hospital of Henan University of Science and Technology,Henan Key Laboratory of Epigenetics,Luoyang 471023,China;Institute of Oncology Precision Medicine and Pathology,Jinan University Medical College,Guangzhou 510632,China)

机构地区：[1]河南科技大学医学院,临床医学院,第一附属医院,肿瘤医院,河南省肿瘤表观遗传重点实验室,河南洛阳471023 [2]暨南大学医学院肿瘤精准医学和病理研究所,广东广州510632

出　　处：《食管疾病》2019年第4期43-46,共4页Journal of Esophageal Diseases

基　　金：洛阳市科技计划医疗卫生项目(1813003A)

摘　　要：目的分析GSK3β对食管癌KYSE450细胞迁移、侵袭和克隆增殖方面的调控作用。方法采用Wound-healing、Transwell、平板克隆形成实验等方法检测GSK3β对食管癌KYSE450细胞迁移、侵袭和克隆增殖方面调控作用的影响,并分析其调控作用及意义。结果在食管癌KYSE450细胞系中,对GSK3β进行下调时,其迁移、侵袭及克隆增殖方面恶性生物学行为显著减弱,差异有统计学意义(P<0.05)。结论GSK3β高表达能显著促进食管癌KYSE450细胞的恶性生物学行为,抑制GSK3β表达可能成为食管癌预后的评价指标,并为食管鳞癌的治疗提供新的靶点。Objective To analyze the effects of GSK3βon the regulation of migration,invasion and clonal proliferation of esophageal cancer KYSE450 cells.Methods Wound healing,Transwell,plate clone formation experiment and other methods were used to detect the influence of GSK3βon malignant biological behavior of esophageal cancer KYSE450 cells,and to analyze its regulatory role and significance.Results In the KYSE450 cell line of esophageal carcinoma,when the GSK3βwas down-regulated,its malignant biological behaviors in terms of migration,invasion and clone proliferation were significantly reduced,and the difference was statistically significant(P<0.05).Conclusion High expression of GSK3βcan significantly promote the malignant biological behavior of esophageal cancer KYSE450 cells.Inhibition of GSK3βexpression may be a prognostic indicator of esophageal squamous cell carcinoma and provide a new target for the treatment of esophageal squamous cell carcinoma.

关 键 词：食管癌 GSK3Β 迁移 侵袭 克隆 

分 类 号：R734.2[医药卫生—肿瘤]