不同分期白塞病葡萄膜炎患者外周血白细胞变化  

Changes of peripheral blood leukocyte in Behçet uveitis patients with different stages

作　　者：刘新书[1,3] 黄春梅 李永哲[2] 张美芬[1] Liu Xinshu;Huang Chunmei;Li Yongzhe;Zhang Meifen(Department of Ophthalmology,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100730,China;Department of Clinical Laboratory,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100730,China;Department of Ophthalmology,The Fourth People's Hospital of Shenyang,Shenyang 110031,China)

机构地区：[1]中国医学科学院北京协和医学院,北京协和医院眼科,100730 [2]中国医学科学院北京协和医学院,北京协和医院检验科,100730 [3]沈阳市第四人民医院眼科,110031

出　　处：《中华实验眼科杂志》2019年第12期971-975,共5页Chinese Journal Of Experimental Ophthalmology

基　　金：国家自然科学基金项目(81770917)。

摘　　要：目的分析白塞病葡萄膜炎(BU)不同疾病分期患者外周血各类白细胞的变化。方法采用病例对照研究设计。收集2015年7月至11月间就诊于北京协和医院眼科的BU患者,其中炎症活动期20例,炎症稳定期21例。炎症活动期BU患者经糖皮质激素和/或免疫抑制剂治疗后转为炎症好转期10例。同期收集于北京协和医院体检的健康对照者82人。抽取受检者外周血并采用HematoFlow血液流式细胞分析平台检测分析12类白细胞分布情况。观察和比较BU患者炎症活动期、炎症好转期、炎症稳定期及健康对照外周血各类白细胞百分比。结果炎症活动期BU组、炎症稳定期BU组和健康对照组外周血成熟嗜中性粒细胞百分比、嗜酸性粒细胞百分比、嗜碱性粒细胞百分比、B淋巴细胞百分比、非细胞毒性T和NK淋巴细胞百分比、颗粒性T和NK淋巴细胞百分比、原始T淋巴细胞百分比、原始B淋巴细胞和未成熟粒细胞百分比总体比较,差异均有统计学意义(F=42.324、10.220、8.660、11.254、29.795、31.305、23.742、27.738和34.638,均P<0.001)。炎症活动期BU组及炎症稳定期BU组患者外周血成熟嗜中性粒细胞百分比分别为(73.10±10.21)%和(62.40±12.09)%,均高于健康对照组的(54.95±6.07)%,炎症活动期BU组患者外周血成熟嗜中性粒细胞百分比高于炎症稳定期BU组,差异均有统计学意义(均P<0.05)。炎症活动期BU组和炎症稳定期BU组患者外周血非细胞毒性T和NK淋巴细胞及颗粒性T和NK淋巴细胞百分比均低于健康对照组,且炎症活动期BU组患者非细胞毒性T和NK淋巴细胞及颗粒性T和NK淋巴细胞百分比低于炎症稳定期BU组,差异均有统计学意义(均P<0.05)。炎症好转期BU组患者治疗后外周血未成熟粒细胞百分比较治疗前明显升高,差异有统计学意义(t=-2.469,P=0.036)。结论BU患者炎症活动期外周血成熟嗜中性粒细胞增加,对于监测BU炎症活动有参考意义�Objective To analyze the changes of peripheral blood leukocyte in patients with Behçet uveitis(BU)at different stages.Methods Case control study was performed.Twenty active stage BU patients and 21 quiet stage BU patients were enrolled from July to November 2015 in Peking Union Medical College Hospital.Ten active stage BU patients treated with glucocorticoid and/or immunosuppressive agents were served as improvement stage BU patients.Meanwhile,82 healthy controls were collected from the physical examination center.Peripheral blood was obtained and then analyzed by using Hematoflow method.The percentages of leukocytes in peripheral blood of different stage BU patients were compared.This study was approved by the Ethics Committee of Peking Union Medical College Hospital(ZS-1341)and all participants signed informed consent.Results The percentage of mature neutrophils,eosinophils,basophils,B lymphocytes,non-cytotoxic T and NK lymphocytes,granular T and NK lymphocytes,T blasts,B blasts and immature granulocytes were all significantly different among active stage BU group,quiet stage BU group and healthy control group(F=42.324,10.220,8.660,11.254,29.795,31.305,23.742,27.738 and 34.638,all at P<0.001).The percentage of mature neutrophils in active stage BU group and quiet stage BU group were(73.10±10.21)%and(62.40±12.09)%,which were significantly higher than(54.95±6.07)%in healthy control group.The percentage of mature neutrophils in active stage BU group was significantly higher than that in quiet stage BU group(P<0.05).The percentages of non-cytotoxic T,NK lymphocytes,granular T and NK lymphocytes in active stage BU group and quiet stage BU group were significantly lower than that in healthy control group,the percentage of non-cytotoxic T,NK lymphocytes and granular T,NK lymphocytes in active stage BU group were significantly lower than those in quiet stage BU group(all at P<0.05).The percentage of immature granulocytes after treatment was significantly higher than that before treatment in improvement stage BU gro

关 键 词：白塞病 葡萄膜炎 外周血白细胞 嗜中性粒细胞 

分 类 号：R73[医药卫生—肿瘤]