超高效液相-串联质谱法测定食品中罂粟壳成分  

Determination of Pericarpium Papaveris in food by ultra high performance liquid chromatography-tandem mass spectrometry

作　　者：张子臣 杜国辉[2] 曹宁 刘建洋 朱爱国[4] ZHANG Zi-Chen;DU Guo-Hui;CAO Ning;LIU Jian-Yang;ZHU Ai-Guo(Shandong Standard Testing Technology Co.,Ltd.,Jinan 250100,China;Shandong Institute of Commerce and Technology,Jinan 250100,China;Jinan Fruit Research Institute All China Federadtion of Supply and Marketing Co Operatives,Jinan 250100,China;Shandong Academy of Agricultural Sciences Test Base,Jinan 250100,China)

机构地区：[1]山东标准检测技术有限公司,济南250100 [2]山东商业职业技术学院,济南250100 [3]中华全国供销合作总社济南果品研究院,济南250100 [4]山东省农业科学院试验基地,济南250100

出　　处：《食品安全质量检测学报》2019年第23期8111-8114,共4页Journal of Food Safety and Quality

摘　　要：目的建立超高效液相-串联质谱法测定食品中罂粟壳成分的分析方法。方法罂粟壳经乙腈提取后,经PCX固相萃取柱净化,选择BEH HILIC柱(粒径1.7μm,2.1 mm×100 mm),以含0.1%甲酸的10 mmol/L乙酸铵溶液和甲酸乙腈溶液(0.1%)作为流动相进行梯度洗脱,在0.3mL/min流速下,以多反应监测(multiple reaction monitoring,MRM)扫描模式进行检测,外标法定量。结果吗啡、可待因在25~250μg/kg添加浓度范围内,回收率为68.30~80.81%。罂粟碱、那可丁、蒂巴因在0.5~2.5μg/kg添加浓度范围内,回收率为68.34%~78.91%。本方法罂粟碱、那可丁、蒂巴因、吗啡和可待因定量限分别为0.5、0.5、0.5、25、25μg/kg。结论该方法简便、快速、可靠,可用于各种食品中罂粟壳生物碱的快速筛查与确证。Objective To establish a method of ultra-high performance liquid chromatography-tandem mass spectrometry for the determination of Pericarpium papaveris in food.Methods After extraction with acetonitrile,Pericarpium papaveris was purified by PCX solid phase extraction column.The liquid was performed on BEH HILIC(1.7μm,2.1 mm×100 mm)with gradient elution of 10 mmol/L ammonium acetate solution(containing 0.1%formic acid)and acetonitrile(containing 0.1%formic acid),and the flow rate was 0.3 mL/min.The sample was detected in multiple reaction monitoring(MRM)scan mode,and quantified by external standard method.Results The recoveries rate of morphine,codeine in the range of 25-250μg/kg was 68.30%-80.81%.The recoveries rate of papaverine,nacodin and thebaine in the range of 0.5-2.5μg/kg was 68.34%-78.91%.The quantitative limits of papaverine,nacodin,thebaine,morphine and codeine of the method were0.5,0.5,0.5,25,25μg/kg.Conclusion This method is simple,fast and reliable,which can be used for rapid screening and confirmation of poppy shell alkaloids in various foods.

关 键 词：罂粟壳 罂粟碱 吗啡 前处理方法 液相色谱质谱联用法 

分 类 号：R28[医药卫生—中药学]