小檗碱调节翼状胬肉成纤维细胞增殖能力的机制研究  

Mechanism of berberine on the proliferation of pterygium fibroblasts

作　　者：陈静[1] 李威[2] 匡洪影[3] 樊锐锋[4] 孙河[5] Jing Chen;Wei Li;Hong-Ying Kuang;Rui-Feng Fan;He Sun(Second Department of Surgery,the First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,Heilongjiang Province,China;Pharmacology Laboratory of Chinese Medicine,the First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,Heilongjiang Province,China;Second Department of Gynaecology,the First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,Heilongjiang Province,China;Department of Medicinal Botany,College of Pharmacy,Heilongjiang University of Chinese Medicine,Harbin 150040,Heilongjiang Province,China;Department of Ophthalmology,the First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin 150040,Heilongjiang Province,China)

机构地区：[1]黑龙江中医药大学附属第一医院外二科,中国黑龙江省哈尔滨市150040 [2]黑龙江中医药大学附属第一医院中药药理实验室,中国黑龙江省哈尔滨市150040 [3]黑龙江中医药大学附属第一医院妇二科,中国黑龙江省哈尔滨市150040 [4]黑龙江中医药大学药学院,中国黑龙江省哈尔滨市150040 [5]黑龙江中医药大学附属第一医院眼科,中国黑龙江省哈尔滨市150040

出　　处：《国际眼科杂志》2020年第1期21-26,共6页International Eye Science

基　　金：国家自然科学基金青年科学基金项目(No.81503610);黑龙江中医药大学优秀创新人才基金项目(No.2018jc05)~~

摘　　要：目的:探讨小檗碱对体外培养翼状胬肉成纤维细胞增殖能力的影响及可能的作用机制。方法:通过对手术获取的翼状胬肉组织进行培养,获取翼状胬肉成纤维细胞。采用不同终浓度(0、20、40、80μmol/L)小檗碱对细胞进行诱导,检测小檗碱诱导后翼状胬肉成纤维细胞凋亡水平、线粒体膜电位和凋亡相关因子mRNA与蛋白表达水平变化。结果:小檗碱能够以剂量依赖的方式提高体外培养翼状胬肉成纤维细胞线粒体去极化水平、细胞凋亡比率、促凋亡基因Bax和Bad mRNA与蛋白的表达水平,降低Bcl-2 mRNA和蛋白的表达水平。结论:小檗碱可能通过提高体外培养翼状胬肉细胞线粒体去极化水平诱导胬肉细胞发生凋亡。AIM:To investigate the effect of berberine on proliferation of in vitro pterygium fibroblasts and its possible mechanism.METHODS:Pterygium fibroblast obtained from cultured pterygium tissue after surgical excision.Pterygium fibroblasts cells apoptosis level,mitochondrial membrane potential and apoptosis-related factors mRNA and protein expression levels were detected after the induction of berberine with different final concentrations(0,20,40,80μmol/L).RESULTS:Berberine increased the mitochondrial depolarization level,apoptosis rate,expression level of pro-apoptotic gene Bax,Bad mRNA and protein,and decreased the expression level of Bcl-2 gene mRNA and protein in vitro pterygium fibroblasts cells in a dose-dependent manner.CONCLUSION:Berberine may induce in vitro cultured pterygium cell apoptosis by increasing mitochondrial depolarization.

关 键 词：翼状胬肉 小檗碱 细胞凋亡 线粒体去极化 

分 类 号：R73[医药卫生—肿瘤]