抑制COTE1表达对多柔比星诱导的肝癌细胞凋亡的影响  

Effect of COTE1 down-regulation on the apoptosis of hepatocarcinoma cells induced by doxorubicin

作　　者：朱津 张海[1] 徐三荣[1] ZHU Jin;ZHANG Hai;XU San-rong(Department of General Surgery, the Affiliated Hospital of Jiangsu University, Zhenjiang Jiangsu 212001, China)

机构地区：[1]江苏大学附属医院普外科

出　　处：《江苏大学学报（医学版）》2019年第6期481-484,共4页Journal of Jiangsu University：Medicine Edition

摘　　要：目的:观察COTE1基因对多柔比星诱导肝癌细胞PLC/PRF/5凋亡的影响。方法:利用COTE1 shRNA慢病毒抑制肝癌PLC/PRF/5细胞中COTE1 mRNA的表达,通过实时荧光定量PCR及蛋白质印迹法分别检测COTE1 mRNA及蛋白表达水平,采用CCK-8法检测多柔比星对肝癌细胞的增殖抑制率;通过流式细胞术检测细胞经多柔比星处理后的凋亡情况。结果:经慢病毒感染后肝癌细胞COTE1 mRNA及蛋白表达下调; CCK-8结果显示,多柔比星对慢病毒感染后肺癌细胞的增殖抑制率增高;流式细胞术结果显示,多柔比星促进肝癌细胞凋亡,而抑制COTE1表达能增强多柔比星对肝癌细胞的凋亡诱导作用。结论:抑制COTE1基因能增强多柔比星对肝癌PLC/PRF/5细胞的凋亡诱导作用。Objective: To investigate the effect of COTE1 gene on hepatocarcinoma cell line PLC/PRF/5 apoptosis induced by doxorubicin(DOX). Methods: The expression level of COTE1 mRNA and protein were detected by qPCR or Western blotting after transfected with COTE1 shRNA lentivirus.CCK-8 assay was used to detect the inhibition rate of DOX on the proliferation of HCC cells.The apoptosis rate of HCC cells treated with DOX was detected by flow cytometry. Results: qPCR and Western Blotting results showed the expression of COTE1 was down-regulated after transfection with lentivirus and CCK-8 results showed the inhibitory effect of DOX was increased. Flow cytometry results showed that DOX could promote the apoptosis rate, while inhibition of the expression of COTE1 can enhance the apoptosis rate.Conclusion: Inhibition of COTE1 could enhance the sensitivity of PLC/PRF/5 cells to doxorubicin by enhancing apoptosis.

关 键 词：COTE1 肝癌细胞 多柔比星 凋亡 

分 类 号：R730.53[医药卫生—肿瘤]