不同水体硬度条件下Cu2+对不同生长阶段斑马鱼的毒性  

TOXICITY OF COPPER(Cu2+) TO ZEBRAFISH AT DIFFERENT LIFE STAGES UNDER DIFFERENT WATER HARDNESS

作　　者：廖伟 刘大庆 冯承莲[1,2] 金小伟 刘娜[1] 白英臣[1,2] 吴丰昌[1] LIAO Wei;LIU Da-qing;FENG Cheng-lian;JIN Xiao-wei;LIU Na;BAI Ying-chen;WU Feng-chang(State Key Laboratory of Environmental Criteria and Risk Assessment,Chinese Research Academy of Environmental Sciences,Beijing 100012,China;Key Laboratory of Poyang Lake Environment and Resource Utilization,Ministry of Education,School of Environmental and Chemical Engineering,Nanchang University,Nanchang 330031,China;Jiangxi Irrigation Experiment Central Station,Nanchang 330201,China;China National Environmental Monitoring Center,Beijing 100012,China)

机构地区：[1]中国环境科学研究院环境基准与风险评估国家重点实验室,北京100012 [2]南昌大学资源环境与化工学院鄱阳湖环境与资源利用教育部重点实验室,南昌330031 [3]江西省灌溉试验中心站,南昌330201 [4]中国环境监测总站,北京100012

出　　处：《环境工程》2019年第12期71-74,166,共5页Environmental Engineering

基　　金：水体污染控制与治理科技重大专项(2017ZX07301005-001)

摘　　要：以斑马鱼为研究对象,开展了不同硬度水质条件下Cu^2+对不同生长阶段斑马鱼的毒性研究。结果表明:同等水质条件下,Cu^2+对不同阶段斑马鱼的毒性顺序为成鱼>幼鱼>仔鱼;同一阶段的受试斑马鱼,水质硬度与Cu^2+对斑马鱼毒性呈负相关(R 2>0.90),硬度为50,125,250 mg/L时,96h-LC 50几何平均值分别为0.20,0.24,0.35 mg/L。通过Cu^2+对斑马鱼仔鱼、幼鱼、成鱼和全生命周期平均急性毒性与水体硬度对数拟合计算得到的硬度斜率分别为0.301、0.471、0.279和0.359。研究结果可为重金属的生物有效性和生态风险评估提供理论依据。Acute toxicity of copper(Cu^2+)at different life stages of zebrafish under different hardness(as CaCO 3)was studied in this work.The results showed that the acute toxicity of copper to zebrafish at different life stages was different,and the 96 h-LC 50 value of copper were in the sequence of larvae-stage-zebrafish>juvenile-stage-zebrafish>adult-stage-zebrafish;as the hardness increased,the acute toxicity of copper to Zebrafish was decreased.There was a significant linear correlation between the acute toxicity of copper to zebrafish and its hardness(R 2>0.90).The geometric mean values of 96h-LC 50 for copper at different life stages of zebrafish were 0.20,0.24,0.35 mg/L in a series of hardness of 50,125,250 mg/L respectively;the hardness slope of toxicity of copper to the larvae-stage,juvenile-stage,adult-stage and the whole-life-stage of zebrafish was 0.301,0.471,0.279 and 0.359,namely.In conclusion,the results could provide theoretical basis for the bioavailability and ecological risk assessment on heavy metals.

关 键 词：不同生长阶段 斑马鱼 硬度斜率 急性毒性 水质基准 

分 类 号：X171.5[环境科学与工程—环境科学] X52