Expression of PD1 and BTLA on the CD8^+T Cell and γδT Cell Subsets in Peripheral Blood of Non-Small Cell Lung Cancer Patients  

非小细胞肺癌患者外周血CD8^+和γδT细胞亚群表面PD1和BTLA的表达（英文）

作　　者：鲍轶 莫娟芬 吴加元 曹晨曦 

机构地区：[1]嘉兴市第二医院中心实验室 [2]嘉兴市第二医院肿瘤科

出　　处：《Chinese Medical Sciences Journal》2019年第4期248-255,共8页中国医学科学杂志（英文版）

基　　金：Fund supported by the Healthcare Technology Plan of Zhejiang Provincial Health Bureau(No.2016KYB292);the Technology Plan of Science and Technology Bureau of Jiaxing,Zhejiang province(No.2016AY23054)~~

摘　　要：Objective To investigate the expression and regulation of programmed cell death protein 1(PD1),B lymphocyte and T lymphocyte attenuator(BTLA)in peripheral blood of patients with non-small cell lung cancer(NSCLC);to examine the correlation of the mRNA levels between PD and BTLA in NSCLC.Methods Flow cytometry was used to detect the expression of PD1 and BTLA on the surfaces of CD8^+T cells andγδ+T cells in the peripheral blood samples collected from 32 in-patients with stage IV NSCLC and 30 healthy individuals.We compared the expression of PD1 and BTLA on the surfaces ofγδ+T cells in the NSCLC patients with bone metastasis before and after the treatment of zoledronic acid.The correlations of PD1 and BTLA,as well as their ligands were analyzed using Pearson correlation analysis with the cBioPortal data platform.Results The frequency of PD1 on the surfaces of CD8^+T cells was significantly higher than that of theγδT cells in both healthy controls(t=2.324,P=0.024)and NSCLC patients(t=2.498,P=0.015).The frequency of PD1 on CD8^+T cells,rather than onγδ+T cells,was significantly upregulated in advanced NSCLC patients compared with that in healthy controls(t=4.829,P<0.001).The PD1+BTLA+γδT cells of the healthy controls were significantly lower than that of the NSCLC patients(t=2.422,P=0.0185).No differences in percentage of PD1+γδ+and BTLA+γδ+T cells were observed in 7 NSCLC patients with bone metastasis before and after zoledronic acid treatment.PD1 was positively correlated with BTLA in both lung adenocarcinoma(r=0.54;P<0.05)and lung squamous cell carcinoma(r=0.78;P<0.05).Conclusions The upregulation of co-inhibitory molecules occurs on the surfaces of both CD8^+T cells andγδT cells in advanced NSCLC,suggesting that these molecules were involved in regulating the inactivation of CD8^+T cells andγδ+T cells,immune escape and tumor invasion.Objective To investigate the expression and regulation of programmed cell death protein 1(PD1), B lymphocyte and T lymphocyte attenuator(BTLA) in peripheral blood of patients with non-small cell lung cancer(NSCLC); to examine the correlation of the m RNA levels between PD and BTLA in NSCLC. Methods Flow cytometry was used to detect the expression of PD1 and BTLA on the surfaces of CD8^+ T cells and γδ+ T cells in the peripheral blood samples collected from 32 in-patients with stage IV NSCLC and 30 healthy individuals. We compared the expression of PD1 and BTLA on the surfaces of γδ+ T cells in the NSCLC patients with bone metastasis before and after the treatment of zoledronic acid. The correlations of PD1 and BTLA, as well as their ligands were analyzed using Pearson correlation analysis with the c Bio Portal data platform.Results The frequency of PD1 on the surfaces of CD8^+ T cells was significantly higher than that of the γδT cells in both healthy controls(t = 2.324, P = 0.024) and NSCLC patients(t = 2.498, P = 0.015). The frequency of PD1 on CD8^+ T cells, rather than on γδ+ T cells, was significantly upregulated in advanced NSCLC patients compared with that in healthy controls(t = 4.829, P < 0.001). The PD1+ BTLA+γδT cells of the healthy controls were significantly lower than that of the NSCLC patients(t = 2.422, P = 0.0185). No differences in percentage of PD1+γδ+ and BTLA+γδ+ T cells were observed in 7 NSCLC patients with bone metastasis before and after zoledronic acid treatment. PD1 was positively correlated with BTLA in both lung adenocarcinoma(r = 0.54; P < 0.05) and lung squamous cell carcinoma(r = 0.78; P < 0.05).Conclusions The upregulation of co-inhibitory molecules occurs on the surfaces of both CD8^+ T cells and γδT cells in advanced NSCLC, suggesting that these molecules were involved in regulating the inactivation of CD8^+ T cells and γδ+ T cells, immune escape and tumor invasion.

关 键 词：CD8^+T cell γδT cell programmed cell death protein 1 B and T lymphocyte attenuator non-small cell lung cancer 

分 类 号：R73[医药卫生—肿瘤]