单眼形觉剥夺小鼠闪光视觉诱发电位与视知觉行为学的改变  

Changes of Flash Visual Evoked Potential and Visual Perception Behavior in Monocular-deprivation Mice

作　　者：丁素真 李兰[1] 朱贺飞[1] 李少敏 董洁[1] DING Su-zhen;LI Lan;ZHU He-fei;LI Shao-min;DONG Jie(Dept.of Ophthalmology,Kunming First People’s Hospital,Kunming Yunnan 650051;Dept.of Neurology,Brigham and Women's Hospital,Harvard Medical School,Boston,MA,USA)

机构地区：[1]昆明市第一人民医院眼科,云南昆明650051 [2]美国哈佛大学附属布莱根妇女儿童医院神经科,美国波士顿

出　　处：《昆明医科大学学报》2019年第12期34-38,共5页Journal of Kunming Medical University

基　　金：云南省科技计划基金资助项目(2016IA022)

摘　　要：目的探究单眼形觉剥夺小鼠闪光视觉诱发电位与视知觉行为学的改变。方法选择健康3周龄小鼠100只,检测fVEP;将35只小鼠随机分为单眼形觉剥夺组(25只)和正常对照组(10只)。单眼形觉剥夺组小鼠随机选择1只眼行褥式缝合,7 d后分别检测小鼠右眼及左眼fVEP,随后将单眼形觉剥夺组小鼠行反向缝合,将对照组小鼠单眼缝合,进行视知觉行为学实验。结果 (1)统计得出100只正常小鼠(200只眼)的fVEP N1波、P1波潜伏期及N1-P1振幅范围;(2)单眼形觉剥夺7 d后,部分小鼠剥夺眼较未剥夺眼fVEP的N1-P1振幅降低,差异有统计学意义(P<0.05);(3)在单眼形觉剥夺组中,fVEPN1-P1波振幅下降的小鼠视知觉行为学完成时间较对照组长,差异有统计学意义(P<0.05);N1-P1波振幅未下降的小鼠视知觉行为学完成时间较对照组长,差异无统计学意义(P>0.05)。结论验证了fVEP检测单眼形觉剥夺小鼠视功能的稳定可靠的指标是N1-P1波振幅降低;视知觉检测单眼形觉剥夺小鼠视功能结果与fVEP检测一致,二者相辅相成,同时运用这2种方法进行小鼠视功能检测,结果更为准确。Objective To investigate the changes of flash visual evoked potential(fVEP) and visual perception behavior in monocular-deprivation mice. Methods A total of 100 3-week-old mice were used to record fVEP. Twenty-five mice were divided into the monocular-deprivation(MD) group,ten mice were divided into normal control(NC) group. In the MD group, the right eye or left eye were randomly sutured by mattress suture for 7 days. The fVEP in the right eye and left eye of each mouse was detected and then a reverse stitching was performed. In the NC group, the right eye or left eye were randomly sutured. The behavioral experiment of visual perception was conducted in both of the two groups. Results (1) The usual range of fVEP N1 wave, P1 wave latency, and N1-P1 amplitude in 100 mice was determined;(2) After monocular deprivation for 7 days, the amplitudes of fVEP N1-P1 in partial MD eyes were significantly lower than that in non-deprivation eyes P<0.05, the difference was significant.(3) In the MD group,the completion time of visual perception behavior in group with N1-P1 amplitude decrease was longer than that in the NC group,P<0.05 the difference was statistically significant. The completion time of visual perception behavior in group without N1-P1 amplitude decrease was longer than that in the NC group P>0.05,the difference was not statistically significant. Conclusions The stable and reliable indicator in monocular-deprivation mice is the decrease of fVEP N1-P1 amplitude. The results of visual perception behavior of monocular-deprivation mice are consistent with fVEP detection. The two methods are complementary to each other,so the results are more accurate by using both of them.

关 键 词：单眼形觉剥夺 视觉诱发电位 视知觉行为学 小鼠 

分 类 号：R779.7[医药卫生—眼科]