欧李自交不亲和S-RNase基因型鉴定及序列分析  

Identification and Sequence Analysis of S-RNase Genes From Prunus humilis Bunge

作　　者：殷亚蕊 武军凯 马聚泽 王海静 李晓颍[1] 张立彬[1] YIN Yarui;WU Junkai;MA Juze;WANG Haijing;LI Xiaoying;ZHANG Libin(College of Horticulture Science and Technology Hebei Normal University of Science and Technology,Qinhuangdao,Hebei 066004)

机构地区：[1]河北科技师范学院园艺科技学院

出　　处：《北方园艺》2019年第24期28-35,共8页Northern Horticulture

基　　金：河北省科技计划资助项目(15236802D);国家自然科学基金资助项目(31401853)

摘　　要：以欧李4个品种为试验材料,采用同源克隆的试验方法,克隆S-RNase基因并分析其序列特征,确定不同品种基因型,以期为欧李杂交育种亲本选择提供参考依据。结果表明:克隆鉴定得到5个新的S-RNase基因,编码氨基酸168~172个,相对分子质量为19.87~20.34 kDa,等电点(PI)为9.60~9.73,以丝氨酸磷酸化为主;二级结构主要以α-螺旋和不规则卷曲为主;同源性比对和结构分析表明,5个新的S-RNase均属于RNase T2基因家族,推导的氨基酸序列包含5个保守区(C1、C2、C3、RC4和C5)和1个高变区(HV),具有与李属、梨属、苹果属S-RNase相似的保守结构;进化分析显示欧李S-RNase与李属果树S-RNase聚类在一起,亲缘关系较近。The S-RNase gene was cloned from four cultivars of Prunus humilis by homologous cloning and its sequence characteristics were analyzed to determin the genotypes of different cultivars.It could provide reference for parents selection in Prunus humilis hybrid breeding.The results showed that five new S-RNase alleles were cloned and identified,encoding 168-172 amino acids with relative molecular weight of 19.87-20.34 kDa and theoretical PI was 9.60-9.73,whose phosphorylation was mainly serine.The main components of their secondary structure were alpha-helix and irregular curl.Homology comparison and structural analysis revealed that all the five S-RNases belonged to RNase T2 family.The deduced amino acid sequence consisted of five conservative regions(C1,C2,C3,RC4 and C5)and one highly variable region(HV),which had a conservative structure similar to S-RNase of Prunus,Pyrus and Malus.

关 键 词：欧李 S-RNASE基因 克隆 自交不亲和性 

分 类 号：S662.9[农业科学—果树学]