蟾皮提取物固体脂质纳米粒的冻干工艺研究  

Study on the freeze-drying technology of solid lipid nanoparticles from toad skin extract

作　　者：张磊 蔡晓瑶 张莉[3] 向晓辉 徐学君 ZHANG Lei;CAI Xiaoyao;ZHANG Li;XIANG Xiaohui;XU Xuejun(Department of Pharmacy,Armed Police Characteristic Medical Center,Tianjin 300162,China;The First Mobile Command of PAP,Shijiazhuang,Hebei 050800;Logistics University of PAP,Tianjin 300162,China;Department of Hepatopancreatobiliary and Splenic Medicine,Armed Police Characteristic Medical Center,Tianjin 300162,China;Department of Pharmacy,Anhui Provincial Corps Hospital of PAP,Hefei,Anhui 230000,China)

机构地区：[1]武警特色医学中心药剂科,天津300162 [2]武警第一机动总队,河北石家庄050800 [3]武警后勤学院,天津300162 [4]武警特色医学中心肝胆胰脾中心,天津300162 [5]武警安徽总队医院药剂科,安徽合肥230000

出　　处：《安徽医药》2020年第1期78-82,共5页Anhui Medical and Pharmaceutical Journal

基　　金：天津市自然科学基金（17JCYBJC26700)

摘　　要：目的对蟾皮提取物固体脂质纳米粒冻干工艺进行考察。方法以外观、复溶性、颗粒形态为指标,筛选最佳的冻干保护剂和冻干工艺。结果选择乳糖、甘露醇、蔗糖、磷脂(300 mg)比例为1.5∶2∶1.5∶1作为混合保护剂,优化后的冻干工艺为-40℃下预冻8 h,-45℃下升华干燥20 h、25℃下解析干燥4 h。结论通过优化保护剂和冻干工艺,制备的冻干固体脂质纳米粒外观饱满,复溶性好,稳定性得到显著提高。Objective To optimize solid lipid nanoparticles freeze⁃drying technology and prescription.Method The freeze⁃drying technology and types of freeze⁃drying protective additive were selected based on appearance,re⁃dissolution and microparticles mor⁃phous.Results The optimal freeze⁃drying protective additive was mixed by lactose,mannitol,sucrose,phospholipid(300mg)at 1.5∶2∶1.5∶1 ratio.After optimized,the freeze⁃drying process was prefreezing 8h under-40℃,sublimation⁃drying 20h under-45℃,de⁃sorption drying 4h under 25℃.Conclusion By optimizing the protective agent and lyophilization process,the freeze⁃drying solid lipid nanoparticles were prepared with plump appearance,a good re⁃dissolution and significantly improved stability.

关 键 词：华蟾蜍毒素/分离和提纯 纳米球 冷冻干燥法 药物稳定性 包封率 冻干保护剂 正交试验 

分 类 号：TS2[轻工技术与工程—食品科学与工程]