响应面法优化多穗石柯总黄酮提取工艺及抗氧化活性研究  

Optimization of Extraction Process and Antioxidant Activity of Total Flavonoids from Lithocarpus polystachyrus Rehd by Response Surface Methodology

作　　者：唐健民 朱成豪 高丽梅[1] 熊雅兰 邹蓉[1] 熊忠臣[1] 蒋运生[1] TANG Jian-min;ZHU Cheng-hao;GAO Li-mei(Guangxi Key Laboratory of Karst Plant Conservation and Restoration Ecology,Guangxi Institute of Botany,Chinese Academy of Sciences,Guangxi Zhuang Autonomous Region,Guilin,Guangxi 541006)

机构地区：[1]广西喀斯特植物保育与恢复生态学重点实验室广西壮族自治区中国科学院广西植物研究所

出　　处：《安徽农业科学》2020年第1期181-185,共5页Journal of Anhui Agricultural Sciences

基　　金：中央引导地方科技发展专项(桂科ZY1949013);广西科技基础和人才专项(桂科AD17129022);广西植物研究所基本业务费(桂植业18013,桂植业18014,19002);河池市科技攻关项目(河科AB198807)

摘　　要：[目的]优化多穗石柯总黄酮的提取工艺及其抗氧化活性的研究。[方法]运用单因素和响应面试验相结合,通过超声波辅助对其总黄酮的提取进行优化,利用响应面分析法考察料液比、提取温度、提取时间对其总黄酮含量的影响,并进行抗氧化活性分析。[结果]多穗石柯总黄酮最佳提取条件为超声功率328.8 W、超声频率35 kHz、乙醇浓度60%、料液比1∶41.2(g∶mL)、提取温度63.2℃、提取时间37.8 min,总黄酮提取率为19.18%。抗氧化活性试验结果表明,当黄酮浓度为2 mg/mL时,对·OH、DPPH·和O^-2·的最大清除率分别为11.70%、75.86%和32.00%,并具有很好的还原能力。[结论]通过热溶剂浸提超声波辅助提取法提取多穗石柯中的黄酮类化合物,所用时间短、要求温度低、提取率好,方法简单易行,具有明显的优势。该研究采用响应面法确定该方法的最佳提取工艺,以期为多穗石柯产业化及开发新天然抗氧化剂提供理论依据。[Objective]To optimize the extraction process and antioxidant activity of total flavonoids from Lithocarpus polystachyrus.[Method]The combination of single factor and response surface test was used to optimize the extraction of total flavonoids by ultrasonic assist,the effects of solid-liquid ratio,extraction temperature and extraction time on the content of total flavonoids were investigated by response surface analysis,and the antioxidant activity was analyzed.[Result]The optimal extraction conditions of total flavonoids from Lithocarpus polystachyrus were ultrasonic power 328.8 W,ultrasonic frequency 35 kHz,ethanol concentration 60%,solid-liquid ratio 1∶41.2(g∶mL),extraction temperature 63.2℃,extraction.At time 37.8 min,the flavonoid yield was 19.18%.The results of antioxidant activity test showed that when the concentration of flavonoids was 2 mg/mL,the maximum clearance rates of·OH,DPPH·and O^-2·were 11.70%,75.86%and 32.00%,respectively,and had good reducing ability.[Conclusion]Ultrasonic assisted extraction with hot solvent extraction is a simple and effective method for extraction of flavonoids from Lithocarpus polystachyrus.In this study,the response surface method is used to determine the optimal extraction process of this method,so as to provide theoretical basis for the industrialization and the development of new natural antioxidants.

关 键 词：多穗石柯 响应面法 总黄酮 提取工艺 优化 抗氧化活性 

分 类 号：R284[医药卫生—中药学]