基质金属蛋白酶2、基质金属蛋白酶抑制剂2和可溶性CD44在高度近视眼合并原发性开角型青光眼患者房水中的定量研究  

Quantitative study of matrix metalloproteinases-2, tissue inhibitor of metalloproteinases-2 and soluble CD44 in aqueous humor of patients with high myopia and primary open angle glaucoma

作　　者：杜非凡 王雪[1] 吴志鸿 Du Feifan;Wang Xue;Wu Zhihong(Department of Ophthalmology,The Third Medical Center of Chinese PLA General Hospital,Beijing 100039,China)

机构地区：[1]解放军总医院第三医学中心眼科

出　　处：《中华眼科医学杂志（电子版）》2019年第6期335-341,共7页Chinese Journal of Ophthalmologic Medicine(Electronic Edition)

基　　金：武警总部课题(WJHQ2012-23);武警总医院课题(WZ 2015 026)

摘　　要：目的研究房水中可溶性白细胞分化抗原44(sCD44)、基质金属蛋白酶2(MMP-2)、基质金属蛋白酶抑制剂2(TIMP-2)的水平及MMP-2与TIMP-2比值的变化与高度近视眼(HM)合并原发性开角型青光眼(POAG)发病的相关性。方法收集2015年7月至2016年12月期间就诊于解放军总医院第三医学中心的HM、POAG和年龄相关性白内障(CAT)患者47例,根据诊断分为4组,HM合并POAG患者12例(12只眼)作为HMG组;POAG组患者15例(15只眼)作为POAG组;HM患者12例(12只眼)作为HM组;CAT患者8例(8只眼)作为CAT组。以A型超声检查测定眼轴长度。采用双抗体夹心法酶联免疫吸附实验方法测定各组房水中MMP-2、TIMP-2及sCD44的水平。采用单因素方差分析比较各组房水样本之间sCD44、MMP-2、TIMP-2的水平及MMP-2与TIMP-2比值的差异,组间两两比较采用方差分析。采用Pearson相关性检验分析sCD44、MMP-2和TIMP-2之间的相关性及sCD44、MMP-2、TIMP-2与眼轴长度的相关性。结果 HMG组、HM组、POAG组及CAT组房水中sCD44水平分别为(229.86±14.23)ng/l、(227.96±17.91) ng/l、(187.80±14.14) ng/l及(149.74±19.18) ng/l,MMP-2水平分别为(7.83±1.28) ng/ml、(7.78±0.91) ng/ml、(6.10±0.85) ng/ml及(5.42±0.72) ng/ml,TIMP-2水平分别为(128.08±11.41) ng/ml、(115.20±7.44) ng/ml、(91.89±8.26) ng/ml及(63.22±8.69) ng/ml,TIMP-2/MMP-2比值分别为(16.65±2.44)、(14.95±1.55)、(15.32±2.30)及(11.67±0.49),比较差异均有统计学意义(F=53.88,16.05,95.41,9.98;P<0.05)。其中,HMG组、HM组及POAG组房水中sCD44水平较CAT组升高,差异有统计学意义(t=10.75,9.31,5.12;P<0.05);HMG组及HM组房水样本中MMP-2的水平较CAT组升高,差异有统计学意义(t=4.81,6.15;P<0.05);POAG组房水中MMP-2的水平与CAT组比较,差异无统计学意义(t=1.86,P>0.05);HMG组、HM组及POAG组房水中TIMP-2的水平较CAT组升高,差异有统计学意义(t=13.62,14.33,7.45;P<0.05);HMG组、HM组及POAG组中TIMP-2/MMP-2的比值均高于CAT组,差异有Objective The aim of this study was to explore the correlation among the expression of matrix metalloproteinases-2( MMP-2),tissue inhibitor of metalloproteinases-2( TIMP-2),soluble cluster of differentiation antigen 44( s CD44) and TIMP-2/MMP-2 and the morbidity with HM&POAG. Methods Selected 47 objects of high myopia( HM),primary open angle glaucoma( POAG) and age-related cataract( CAT) from July 2015 to December 2016 in the Third Medical Center of Chinese PLA General Hospital. 47 objects were divided into 4 groups according to the diagnosis. 12 patients( 12 eyes) who were diagnosed as HM and POAG were taken as group HMG;15 patients( 15 eyes) who were diagnosed as POAG were taken as group POAG;12 patients( 12 eyes) who were diagnosed as HM were taken as group HM;8 patients( 8 eyes)who were diagnosed as CAT were taken as group CAT. A-scan ultrasonography was used to measure the length of optic axis. Double antibody sandwich enzyme linked immunosorbent assay was used to determine the concentration of MMP-2,TIMP-2 and s CD44 in aqueous humor. Differences among the level of MMP-2,TIMP-2,s CD44 and TIMP-2/MMP-2 of the four groups were verified by one-way ANOVA analysis;the correlation was verified by Pearson correlation test. Results In group HMG,HM,POAG and CAT,the level of s CD44 were( 229. 86 ± 14. 23) ng/l,( 227. 96 ± 17. 91) ng/l,( 187. 80 ± 14. 14) ng/l,( 149. 74 ± 19. 18)ng/l,the level of MMP-2 were( 7. 83 ± 1. 28) ng/ml,( 7. 78 ± 0. 91) ng/ml,( 6. 10 ± 0. 85) ng/ml and( 5. 42 ±0. 72) ng/ml,the level of TIMP-2 were( 128. 08 ± 11. 41) ng/ml,( 115. 20 ± 7. 44) ng/ml,( 91. 89 ±8. 26) ng/ml and( 63. 22 ± 8. 69) ng/ml,the ratio of TIMP-2/MMP-2 were( 16. 65 ± 2. 44),( 14. 95 ±1. 55),( 15. 32 ± 2. 30) and( 11. 67 ± 0. 49),the differences were statistically significant( F =53. 878,16. 051,95. 405,9. 984;P < 0. 05). The difference of the level of s CD44 between each two group of group HMG,HM,POAG and CAT was statistically significant( t = 10. 75,9. 31,5. 12;P < 0. 05). The difference of the level of MMP

关 键 词：基质金属蛋白酶2 基质金属蛋白酶抑制剂2 可溶性白细胞分化抗原44 高度近视合并原发性开角型青光眼 房水 

分 类 号：R778.11[医药卫生—眼科] R775.2[医药卫生—临床医学]