巨杉健康和感病叶片表面微生物组成和多样性比较  

Comparison of microbial compositions and diversities between healthy and infected leaf surfaces of Sequoiadendron giganteum

作　　者：岳雪华 范深厚 杜雪柯 胡嘉琪 张扬[1] 李媛媛[1,3] YUE Xuehua;FAN Shenhou;DU Xueke;HU Jiaqi;ZHANG Yang;LI Yuanyuan(School of Ecological and Environmental Sciences,East China Normal University,Shanghai 200241,China;Research Institute of Forestry,Lichuan,Hubei 445400,China;Institute of Eco-Chongming,Shanghai 200062,China)

机构地区：[1]华东师范大学生态与环境科学学院,上海200241 [2]湖北省利川市林业科学研究所,湖北利川445400 [3]上海市崇明生态研究院,上海200062

出　　处：《福建农林大学学报（自然科学版）》2020年第1期10-17,共8页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家重点研发专项(2017YFC0506002);国家自然科学基金(31670540)

摘　　要：为分析巨杉(Sequoiadendron giganteum)健康叶片和感病叶片表面微生物的组成和多样性异同,以我国引种的巨杉叶片表面微生物类群为研究对象,采用高通量测序对细菌16S rRNA和真菌ITS片段测序,通过OTU数量、多样性指数、鉴定类群等方法,分析叶片表面细菌和真菌组成和多样性的异同.结果表明,健康叶片表面的细菌多样性比感病叶片高,但肠杆菌属(Enterobacter)、金黄杆菌属(Chryseobacterium)和短小杆菌属(Curtobacterium)菌群的含量在感病叶片中占所有细菌群落的比例比健康叶片高;真菌类群在健康叶片表面的多样性比感病叶片丰富,其中葡萄孢属(Botrytis)的卡罗来纳葡萄孢(B.caroliniana)占感病叶片表面真菌总数量的49.60%,远远多于健康叶片所占比例16.10%.健康叶片比感病叶片表面维持了较高的细菌和真菌类群多样性,卡罗来纳葡萄孢真菌在感病叶片表面所占比例远远大于健康叶片,可能与植株感病有关,此结果可为巨杉致病菌的防治提供依据.To compare the microbial compositions and diversities on the healthy and infected leaf surface,we investigated the bacterial and fungal OTUs on healthy and infected leaf surfaces of Sequoiadendron giganteum sampling from the Lichuan Forestry Science Institute of Hubei province,China,using high-throughput sequencing method.The bacterial diversity on the surface of healthy leaves was higher than that of infected leaves,whereas the proportions of Enterobacter(Proteobacteria),Chryseobacterium(Bacteroidetes),and Curtobacterium(Actinobacteria)on the surface of infected leaves were higher than those of healthy leaves.The fungal diversity on the sunface of healthy leaves was higher than that of infected leaves too.The content of Botrytis caroliniana accounted for 49.60%of the total number of fungi on the surface of infected leaves,much more than that of 16.10%in the healthy leaves.In this study,we think that the fungi of B.caroliniana may be related to the disease in S.giganteum since its higher proportion on the infected leaves.This finding is important for the prevention and control of pathogenic microorganism in S.giganteum.

关 键 词：巨杉 细菌 真菌 多样性 致病微生物 

分 类 号：Q143[生物学—生态学]