改良蟾蜍毁脑和脊髓方法的效果评价  

Evaluation of the effect of brain and spinal cord destruction in toads by a modified way

作　　者：朱延河[1] 孟凯 史小莲[3] 王涛[2] 张莉[2] 郭媛[2] 苟玮 胡浩[4] ZHU Yan-he;MENG Kai;SHI Xiao-lian;WANG Tao;ZHANG Li;GUO Yuan;GOU Wei;HU Hao(Institute of Endemic Diseases,School of Public Health of Xi'an Jiaotong University Health Science Center,Xi'an 710061;Department of Physiology and Pathophysiology,School of Basic Medical Sciences of Xi'an Jiaotong University Health Science Center,Xi'an 710061;Department of Pharmacology,School of Basic Medical Sciences of Xi'an Jiaotong University Health Science Center,Xi'an 710061;Basic Medical Experimental Teaching Center,School of Basic Medical Sciences of Xi'an Jiaotong University Health Science Center,Xi'an 710061,China)

机构地区：[1]西安交通大学公共卫生学院地方病研究所,陕西西安710061 [2]西安交通大学基础医学院生理与病理生理学系,陕西西安710061 [3]西安交通大学基础医学院药理学系,陕西西安710061 [4]西安交通大学基础医学实验教学中心,陕西西安710061

出　　处：《西安交通大学学报（医学版）》2020年第1期128-130,共3页Journal of Xi’an Jiaotong University（Medical Sciences）

基　　金：陕西省科学技术研究发展计划项目(No.2019SF-200)~~

摘　　要：目的完善传统教学中使用探针捣毁蟾蜍脑组织和脊髓实验操作技术中的相关内容。方法在传统教学操作内容基础上,增加探针进入颅腔的进针距离和捣毁脑组织及脊髓的易于体会的具体手感标志。按班级对学生进行随机分组,比较常规组和改良组学生首次及第2次操作成功率和花费时间。结果改良组学生捣毁蟾蜍脑组织和脊髓成功率高(首次成功率93.2%vs.72.3%;第2次成功率97.7%vs.85.1%),而且花费时间少[首次操作时间(311.7±89.3)s vs.(511.6±171.1)s;第2次操作时间(161.3±63.5)s vs.(266.0±98.2)s],两组学生的第1、2次成功率和操作时间差异均有统计学意义(P<0.05)。结论传统教学的捣毁蟾蜍脑组织和脊髓内容经过完善后,易于被学生掌握,成功率高,用时少,更符合3R原则,便于教学推广应用。Objective To improve the traditional teaching technique of using probe to destroy the brain tissue and spinal cord of toads.Methods On the basis of the traditional teaching content,the distance of the probe entering the skull cavity and the specific feeling mark of destroying the brain tissue and spinal cord were added.Students were randomly divided into normal group and improved group according to the class.We observed the success rate and time spent on both the first and the second operations in the two groups.Results The success rate of destroying the brain tissue and spinal cord in the improved group was high(93.2%vs.72.3%for the first time).The secondary success rate was 97.7%vs.85.1%,and it took less time(311.7±89.3)seconds vs.(511.6±171.1)seconds for the initial operation,The secondary operation time was(161.3±63.5)seconds vs.(266.0±98.2)seconds,with significant differences between the two groups(P<0.05).Conclusion After the improvement of the content of destroying the brain tissue and spinal cord of toads in traditional teaching,it is easy to be mastered by students,with high success rate,less time spent,greater consistency with the 3R principle,and easier to be popularized and applied in teaching.

关 键 词：蟾蜍 捣毁 脑组织 脊髓 

分 类 号：R-331[医药卫生]