可降解聚酰胺树枝状大分子复合基因载体介导肝癌靶向治疗的应用研究  

Application of compound PAMAM gene vector mediated targeted therapy for liver cancer

作　　者：庄菊花 倪晶 王国玉 夏伟[1] 叶颖[2] Zhuang Juhua;Ni Jing;Wang Guoyu;Xia Wei;Ye Ying(Nuclear Medicine Department,Seventh People′s Hospital of Shanghai University of TCM,Shanghai 200137,China)

机构地区：[1]上海中医药大学附属第七人民医院核医学科,200137 [2]上海中医药大学附属第七人民医院中心实验室,200137

出　　处：《山西医药杂志》2020年第1期7-9,共3页Shanxi Medical Journal

基　　金：上海市卫生健康委员会青年项目(20164Y0097);上海市浦东新区科技经济委员会民生项目(PKJ2016-Y19);上海市浦东新区卫生健康委员会成果转化项目(PW2017F-2);上海中医药大学预算内项目(2019LK044)

摘　　要：目的探讨可降解聚酰胺树枝状大分子(SS-PAMAM)复合基因载体对于人肝癌细胞系HepG2细胞蛋白表达及细胞增殖迁移的影响。方法将人肝癌细胞学HepG2细胞分为观察组和对照组,观察组给予复合型PAMAM基因载体处理,对照组给予空载体处理,蛋白质印迹法检测CDH13蛋白表达,采用四甲基偶氮唑盐(MTT)法和划痕实验检测肿瘤细胞的增殖和迁移能力。结果观察组HepG2细胞CDH13蛋白表达为(0.981±0.019),明显高于对照组的(0.420±0.017),差异有统计学意义(P<0.05);观察组HepG2细胞24 h增殖吸光度值及划痕愈合率分别为(0.554±0.013)和(0.198±0.011),均明显低于对照组(P<0.05)。结论复合型PAMAM基因载体可使人肝癌HepG2细胞的增殖和迁移能力减弱。Objective To investigate the effect of complex PAMAM gene vector on protein expression and cell proliferation of human hepatoma cell line HepG2.Methods HepG2 cells were divided into observation group and control group.The observation group was treated with the composite PAMAM gene vector,the control group was treated with empty vector,and the proliferation and migration ability of tumor cells were detected by MTT assay and scratch test.Results The expression of CDH13 protein of HepG2 cells in the observation group was(0.981±0.019),which was significantly higher than that in the control group(0.420±0.017).The difference was statistically significant(P<0.05).The A value and scratch healing rate of HepG2 cells in the observation group were(0.554±0.013)and(0.198±0.011),respectively,which were significantly lower than those in the control group(P<0.05).Conclusion The composite PAMAM gene vector can attenuate the cell proliferation and migration of human hepatoma HepG2 cells.

关 键 词：肝肿瘤 细胞增殖 细胞运动 PAMAM基因载体 

分 类 号：R73[医药卫生—肿瘤]