氢气联合自然杀伤细胞对肺癌细胞生长的影响  

Influence of hydrogen combined with natural killer cells in lung cancer cell growth

作　　者：夏盛全 任涛[1] 王凯玲[1] 顾霞[1] Xia Shengquan;Ren Tao;Wang Kailing;Gu Xia(Department of Respiratory Medicine,East Hospital,Tongji University,Shanghai 200123,China)

机构地区：[1]同济大学附属东方医院呼吸内科,上海200123

出　　处：《国际呼吸杂志》2019年第24期1863-1869,共7页International Journal of Respiration

基　　金：国家自然科学基金(81673014);上海市优秀学术带头人计划(16XD1403100);宁波市镇海区医学科技计划(2017006)。

摘　　要：目的研究不同浓度氢气联合自然杀伤(NK)细胞是否有助于抑制肺癌细胞生长。方法从非小细胞肺癌患者外周血中分离和培养NK细胞,用20%、40%、60%不同浓度氢气分别处理NK细胞。通过四甲基偶氮唑蓝法检测NK细胞生长曲线、流式细胞仪分析NK细胞周期、酶联免疫吸附试验分析NK细胞分泌炎症因子的变化来评估氢气对NK细胞的增殖能力影响。将NK细胞和A549细胞(肺癌细胞)1∶1加入共培养体系,用20%、40%、60%不同浓度氢气分别处理,通过观察A549细胞单克隆形成能力、划痕分析A549细胞迁移能力、Transwell分析A549细胞侵袭能力来评估氢气联合NK细胞杀瘤能力。结果20%、40%、60%不同浓度的氢气对NK细胞的增殖能力影响有限。在A549细胞单克隆生长能力和细胞迁移能力方面,60%的氢气联合NK细胞有轻度抑制作用,20%、40%浓度的氢气和单独NK细胞作用无差别;Transwell分析细胞侵袭能力提示20%、40%、60%不同浓度氢气联合NK细胞与单独NK细胞对A549细胞侵袭能力无差别。结论60%高浓度氢气联合NK细胞有助于抑制A549细胞单克隆生长和细胞迁移能力,从而抑制肿瘤生长,为今后进一步科学研究和氢气联合NK细胞免疫治疗肺癌奠定了基础。Objective To study whether the different concentrations of hydrogen combined with natural killer(NK)cells can contribute to the inhibition of lung cancer cell growth.Methods NK cells were isolated and cultured from peripheral blood of patients with non-small cell lung cancer.NK cells were treated with 20%,40%,60%hydrogen at different concentrations.NK cell growth curve was measured by methyl thiazolyl tetrazolium assay,NK cell cycle was analyzed by flow cytometry,the secretion of inflammatory factors in NK cells was analyzed by enzyme linked immunosorbent assay,in order to assess the impact of hydrogen on the proliferation of NK cells.NK cells and A549 cells(lung cancer cells)were added to co-culture system with a ratio of 1∶1 treated with different concentrations of hydrogen at the level of 20%,40%,and 60%.The clone-forming,migration,and invasion ability of A549 cells were analyzed to evaluate the killing ability of hydrogen combined with NK cells.Results All the concentrations of hydrogen at the level of 20%,40%,and 60%had little impact on the proliferation of NK cells.In the clone-forming and migration ability of A549 cells,60%of the hydrogen combined with NK cells exhibited a little effect on inhibition,whereas,20%or 40%of the hydrogen had no such effect.Nevertheless,all concentrations of the hydrogen combined with NK cells exhibited no effect on the invasion ability of A549 cells.Conclusions The combination of 60%of the hydrogen and NK cells could inhibit clone-forming and migration ability,as well as tumor growth,which provides a framework for future studies involving combined treatment of 60%of the hydrogen and NK cells for lung cancer.

关 键 词：癌 非小细胞肺 氢气 自然杀伤细胞 细胞增殖 杀瘤能力 

分 类 号：R73[医药卫生—肿瘤]