人参皂苷CK对人骨肉瘤细胞U2-OS增殖及迁移能力的影响  

The effect of ginsenoside CK on the proliferation and migration of human osteosarcoma cells U2-OS

作　　者：陈康 王云飞[1] 骞立刚[1] 梁志兴[1] 孙博[1] 马铮[1] 任俏 CHEN Kang;WANG Yun-fei;QIAN Li-gang;LIANG Zhi-xing;SUN Bo;MA Zheng;REN Qiao(Department of Orthopedics,Affiliated Hospital of Hebei University,Baoding 071000,China;Department of Rheumatism and Immunology,Affiliated Hospital of Hebei University,Baoding 071000,China)

机构地区：[1]河北大学附属医院骨二科,河北保定071000 [2]河北大学附属医院风湿免疫科,河北保定071000

出　　处：《解剖科学进展》2019年第6期630-633,共4页Progress of Anatomical Sciences

基　　金：国家自然科学基金(81100924)

摘　　要：目的探讨人参皂苷CK对人骨肉瘤细胞U2-OS增殖及迁移能力的影响。方法将人骨肉瘤细胞U2-OS分为阴性对照组(Control组)和实验组(CK药物组),MTT方法与CCK-8方法检测细胞活性及增殖能力,细胞划痕实验及Transwell实验检测细胞迁移能力,Western blotting检测侵袭相关蛋白MMP-9及MMP-2的表达。结果CK能够显著降低U2-OS细胞体外活性及生存率(P<0.05);细胞划痕实验及Transwell法证明CK可抑制U2-OS体外迁移能力(P<0.05);CK处理后U2-OS细胞的MMP-9及MMP-2表达水平下调(P<0.05)。结论人参皂苷CK对骨肿瘤细胞U2-OS的抑制作用与下调MMP-9及MMP-2表达水平相关。Objective To investigate the effect of ginsenoside CK on the proliferation and migration of human osteosarcoma cells U2-OS. Methods Human osteosarcoma U2-OS cells were divided into control group and CK drug group. The viability and proliferation of the cells were detected by MTT and CCK-8 respectively, migration was detected by would-healing test and Transwell test. The expression of invasion-related proteins MMP-9 and MMP-2 was detected by Western blot. Results CK significantly reduced the activity and survival rate of U2-OS cells, and inhibited the migration of U2-OS cells, and down-regulated the expression levels of MMP-9 and MMP-2 proteins in U2-OS cells. Conclusion The inhibitory effect of Ginsenoside CK on U2-OS cells is related to the downregulation of MMP-9 and MMP-2 expressions.

关 键 词：人参皂苷CK 骨肉瘤细胞U2-OS 增殖 迁移 

分 类 号：R738.1[医药卫生—肿瘤]