SAHA联合MG132对神经母细胞瘤的抗肿瘤作用及分子机制  

The anti-neuroblastoma effects and molecular mechanisms of SAHA and MG132

作　　者：向莉伟 郑华川 XIANG Li-wei;ZHENG Hua-chuan(Lab.2 of Experimental Oncology,Shengjing Hospital of China Medical University,Shenyang 110004,China)

机构地区：[1]中国医科大学附属盛京医院实验肿瘤学实验室

出　　处：《解剖科学进展》2019年第6期666-669,共4页Progress of Anatomical Sciences

基　　金：国家自然科学基金(81672700);辽宁省自然科学基金(20180530105);沈阳市科技计划项目(18013059);辽宁省特聘教授项目资助

摘　　要：目的研究SAHA和MG132对神经母细胞瘤的抗肿瘤作用及其相关分子机制。方法用SAHA和MG132共同处理SH-SY5Y细胞,检测其增殖、细胞周期、凋亡、迁移、侵袭和表型相关蛋白。结果 SAHA和MG132可协同抑制SH-SY5Y细胞的增殖(P<0.05)、糖代谢(P<0.05)、细胞迁移和侵袭(P<0.05),诱导G1期阻滞和凋亡(P<0.05),并呈现出时间和剂量依赖性。SAHA和/或MG132能上调ING5、PTEN、p53、Caspase-3、Bax、p21和p27的表达,下调14-3-3、MMP-2、MMP-9、c-myc、CyclinD1的表达。结论 SAHA和MG132联合应用可通过抑制增殖、迁移、侵袭、糖代谢和诱导细胞凋亡,有效逆转神经母细胞瘤恶性表型,二者联合使用有现实高效低毒的杀伤效果。Objective To study the anti-tumor effects and related molecular mechanisms of SAHA and MG132 on neuroblastoma cells. Methods SH-SY5Y cells were treated with SAHA and MG132, and then subjected to the examination of proliferation, cell cycle, apoptosis, migration and invasion, as well as phenotype-related proteins. Results SAHA and MG132 synergistically suppressed proliferation of SH-SY5Y cells(P<0.05), the energy metabolism(P <0.05), cell migration and invasion(P<0.05), and induced G1 arrest and apoptosis(P<0.05) in both time-and dose-dependent manners. SAHA and/or MG132 increased the expression of ING5, PTEN, p53, Caspase-3, Bax, p21 and p27, but decreased the expression of 14-3-3, MMP-2, MMP-9, c-myc, and CyclinD1. Conclusion The combination of SAHA and MG132 synergistically and effectively reversed the aggressive phenotypes of neuroblastoma cells by inhibiting proliferation, migration and invasion and glucose metabolism, and inducing apoptosis.

关 键 词：SAHA MG132 神经母细胞瘤 

分 类 号：R739.43[医药卫生—肿瘤]