D-丝氨酸对地卓西平马来酸盐诱导的精神分裂症模型大鼠行为和认知功能的影响  

Effects of D-serine on behavioral and cognitive functions in dizocilpine maleate induced model rats with schizophrenia

作　　者：郑瑞璇[1,2] 程灶火 汪萌芽[1] ZHENG Rui-xuan;CHENG Zao-huo;WANG Meng-ya(Cell Electrophysiology Laboratory,Wannan Medical College,Wuhu 241002,China)

机构地区：[1]皖南医学院细胞电生理研究室,芜湖241002 [2]无锡市儿童医院儿童保健科 [3]无锡市精神卫生中心

出　　处：《临床精神医学杂志》2019年第6期369-372,共4页Journal of Clinical Psychiatry

基　　金：国家自然科学基金项目(31271155)

摘　　要：目的:探讨D-丝氨酸对地卓西平马来酸盐(MK-801)诱导的精神分裂症模型大鼠行为和认知功能的影响。方法:取50只青春期雄性SD大鼠随机均分为5组:MK-801模型(MK)组、MK-801模型+D-丝氨酸低剂量(MK+D1)组、MK-801模型+D-丝氨酸高剂量(MK+D2)组、MK-801模型+利培酮(MK+R)组和生理盐水(NS)组。NS组与其余4组分别给予生理盐水与MK-80114 d造模;造模结束24 h后,NS组与MK组给予生理盐水10 d,MK+D1组、MK+D2组与MK+R组分别接受低剂量与高剂量D-丝氨酸和利培酮干预10 d。在药物干预前后对各组大鼠进行旷场实验、强迫游泳及Y迷宫等行为学实验。结果:与NS组相比,MK-801造模大鼠的旷场实验自主活动及探究行为均增加,强迫游泳实验不动时间延长,Y迷宫实验交替百分率降低(P<0.05或P<0.01)。与MK组相比,药物干预后MK+D1组、MK+D2组与MK+R组的自主活动及探究行为减少,强迫游泳不动时间缩短,Y迷宫实验交替百分率升高(P<0.05或P<0.01)。结论:D-丝氨酸对精神分裂症模型大鼠的阳性症状、阴性症状以及认知损伤中的工作记忆均有一定的改善作用。Objective:To observe the effects of D-serine on the behavioral and cognitive functions of schizophrenic model rats induced by dizocilpine maleate(MK-801). Method:50 male SD rats were randomly assigned into five groups:MK group,MK+D1 group,MK+D2 group,MK+R group and NS group.NS group was exposed to saline,other groups were all exposed to MK-801 for 14 days.After 24 hours,NS group and MK group received normal saline,and the other three groups were treated with low and high D-serine or risperidone for 10 d.All the rats were tested by the open field test,forced swimming test and Y-maze behavior test before and after the drug intervention. Results:Compared with the NS group,locomotor activity and explorative behavior in open-field test and immobility time in the forced swimming test increased,and alternate percentage in Y-maze spontaneous alternation test decreased in the MK-801 model rats(P<0.05 or P<0.01).After medicine intervention,MK+D1,MK+D2 and MK+R groups showed significantly decreased locomotor activity and explorative behavior and immobility time,and increased alternate percentage(P<0.05 or P<0.01),which were different from the MK group. Conclusion:D-serine may have an positive effect on the positive symptoms,negative symptoms and the working memory in cognitive impairment of model rats with schizophrenia.

关 键 词：精神分裂症 行为 认知功能 D-丝氨酸 

分 类 号：R749.3[医药卫生—神经病学与精神病学]