苦荞转录因子FtNAC15基因的克隆及表达分析  

Cloning and Expression of FtNAC15 Transcription Factor in Fagopyrum tataricum

作　　者：黄娟[1] 荣玉萍 孟子烨[1] 唐彬 章洁琼 夏忠敏 陈庆富[1] HUANG Juan;RONG Yu-ping;MENG Zi-ye;TANG Bin;ZHANG Jie-qiong;XIA Zhong-min;CHEN Qing-fu(Research Center of Buckwheat Industry Technology,Guizhou Normal University,Guiyang 550001,China;Guizhou Provincial Agricultural Technology Extension Station,Guiyang 550001,China;Soil and Fertilizer Institute of Guizhou,Guiyang 550001,China)

机构地区：[1]贵州师范大学荞麦产业技术研究中心,贵州贵阳550001 [2]贵州省农作物技术推广总站,贵州贵阳550001 [3]贵州省土壤肥料工作总站,贵州贵阳550001

出　　处：《江西农业大学学报》2019年第6期1183-1191,共9页Acta Agriculturae Universitatis Jiangxiensis

基　　金：国家自然科学基金项目（31760419）;贵州省科技计划项目（黔科合基础[2016]1107、黔科合LH字[2017]7364号，黔科合成果[2019]4252号，黔科合平台人才[2017]5726-19，黔科合LH字[2016]7214号）;2019年省级农业产业发展资金（黔财农[2019]68号）;贵州师范大学资助博士科研项目（11904/0516031）~~

摘　　要：为研究NAC转录因子在苦荞中的功能,从苦荞发育种子中克隆了一个NAC家族基因,其开放阅读框全长1098 bp,编码365个氨基酸,将其命名为FtNAC15。基因结构分析表明:FtNAC15基因由3个外显子和2个内含子组成。氨基酸序列多重比对和进化关系分析表明:FtNAC15蛋白与水稻ONAC003、拟南芥ANAC010和ANAC073亲缘关系较近,属于NAC家族转录因子家族中同一亚组。基因上游启动子序列顺势作用元件分析表明:该基因启动子中的顺式作用元件可以分为5类,即启动子核心元件、节律和光照响应元件、转录因子结合位点、非生物胁迫响应元件和激素响应元件。表达分析表明:FtNAC15基因在种子发育的成熟期和干旱胁迫下上调表达,表明其参与调控荞麦种子发育和响应干旱胁迫。To study the function of NAC transcription factor in Tartary buckwheat,a NAC family gene was cloned from the developing seeds of Fagopyrum tataricum.The length of the open reading frame was 1 098 bp,and the number of amino acids was 365.Hereafter it was named FtNAC15.Gene structure analysis showed that FtNAC15 was composed of three exons and two introns. Multiple amino acid sequence alignment and evolutionary relationship analyses showed that FtNAC15 protein was closely related to ONAC003 in rice,ANAC010 and ANAC073 in Arabidopsis thaliana,and was classified to the same subgroup of NAC transcription factor family.Cis-acting elements in the promoter of FtNAC15 were predicted and divided into five categories:promoter core elements,rhythm and light response elements,transcription factor binding sites,abiotic stress response elements and hormone response elements.Furthermore,expression analysis showed that FtNAC15 was up-regulated in the mature stage of seed development and under drought stress,indicating that it was involved in seed development regulation and drought stress response of Fagopyrum tataricum.

关 键 词：苦荞 NAC转录因子 克隆 表达 

分 类 号：S517[农业科学—作物学]