Vasohibin-1,MACC1和KAI1在浆液性卵巢癌中的表达及其临床意义  

Expression of vasohibin-1, MACC1 and KAI1 proteins in serous ovarian cancer and their clinical significance

作　　者：俞岚[1] 毛旭 焦云杰 宋文庆[1] 王丹娜[1] YU Lan;MAO Xu;JIAO Yunjie;SONG Wenqing;WANG Danna(Department of Pathology,First Affi liated Hospital of Bengbu Medical College,Department of Pathology,Bengbu Medical College,Anhui Key Laboratory of Infection and Immunology;Department of Clinical Medicine,Bengbu Medical College,Bengbu Anhui 233030,China)

机构地区：[1]蚌埠医学院第一附属医院病理科,蚌埠医学院病理学教研室,安徽省感染与免疫重点实验室 [2]蚌埠医学院临床医学系,安徽蚌埠233030

出　　处：《中南大学学报（医学版）》2019年第12期1344-1352,共9页Journal of Central South University ：Medical Science

基　　金：安徽省高校自然科学研究重点项目(KJ2017A224);国家级大学生创新训练项目(201610367015)~~

摘　　要：目的:探讨浆液性卵巢癌中血管生成抑制蛋白-1(vasohibin-1)、结肠癌转移相关蛋白1(metastasis-associated in colon cancer-1,MACC1)和抗癌1号蛋白(KAI1)表达之间的关系及其临床意义。方法:收集124例卵巢浆液性癌(卵巢浆液性癌组)和30例卵巢浆液性囊腺瘤(卵巢浆液性囊腺瘤组)术后组织标本,采用免疫组织化学ElivisionTM法检测所有肿瘤组织中vasohibin-1,MACC1和KAI1蛋白的表达情况。结果:卵巢浆液性癌组vasohibin-1和MACC1蛋白的阳性表达率分别为48.4%和58.1%,均高于卵巢浆液性囊腺瘤组(分别为10.0%和13.3%);而卵巢浆液性癌组KAI1蛋白阳性表达率为33.9%,低于浆液性囊腺瘤组(86.7%),差异均有统计学意义(均P<0.05);3种蛋白的表达与浆液性卵巢癌的病理组织学分级、国际妇产科联盟(Federation International of Gynecology and Obstetrics,FIGO)分期以及盆腔淋巴结转移有关(均P<0.05);KAI1蛋白的表达与vasohibin-1和MACC1的表达呈负相关(r值分别为–0.500和–0.600,均P<0.01),同时,vasohibin-1与MACC1蛋白的表达呈正相关(r=0.518,P<0.01)。Kaplan-Meier生存分析表明:vasohibin-1和MACC1的过表达以及KAI1的低表达均与患者的生存率有关,vasohibin-1和MACC1表达阳性及KAI1表达阴性的患者生存率明显低于vasohibin-1和MACC1表达阴性及KAI1表达阳性的患者(均P<0.05)。多因素分析表明:FIGO分期、vasohibin-1和KAI1蛋白的表达是影响浆液性卵巢癌根治术后患者预后的独立因素(RR值分别为2.185,3.893,0.413;95% CI分别为1.263~3.779,2.190~6.921,0.251~0.681;均P<0.05)。结论:浆液性卵巢癌组织中vasohibin-1和MACC1的表达上调以及KAI1的表达下调与肿瘤的分化程度、临床分期、转移和预后等因素相关,这些指标的联合检测可作为判断浆液性卵巢癌患者肿瘤演进及生存预后的重要指标。Objective:To examine the expression of vasohibin-1,metastasis-associated in colon cancer-1 (MACC1) and KAI1 proteins in serous ovarian cancer and their clinical significance.Methods:In 124 specimens of serous ovarian cancer (serous ovarian cancer group) and 30 specimens of ovarian serous cystadenoma (ovarian serous cystadenoma group),the expression of vasohibin-1,MACC1 and KAI1 protiens were detected by immunohistochemistry ElivisionTM method.Results:In the serous ovarian cancer group,the positive rates of vasohibin-1 and MACC1 proteins were 48.4% and 58.1%,respectively,which were both higher than those in the ovarian serous cystadenoma group (10.0% and 13.3%,respectively);while the positive rate of KAI1 protein in the serous ovarian cancer group was 33.9%,which was lower than that in the ovarian serous cystadenoma group (86.7%),there were significant differences between the 2 groups (all P<0.05).In the serous ovarian cancer group,the expression of the 3 proteins were closely related to the pathological grade,Federation International of Gynecology and Obstetrics (FIGO) stage and pelvic lymph node metastasis (all P<0.05).The KAI1 protein was negatively correlated with the levels of vasohibin-1 and MACC1 (r=–0.500,–0.600,respectively,both P<0.01);while there was a positive correlation between the vasohibin-1 and the MACC1 (r=0.518,P<0.01).Kaplan-Meier survival analysis showed that the over-expression of vasohibin-1,MACC1 and the low-expression of KAI1 protein were related to the survival rates (all P<0.05).Multi-factor analysis showed that the expression of vasohibin-1,KAI1 protein and the FIGO stage were independent prognosis factors for radical operation of serous ovarian cancer (RR=2.185,3.893,0.413;95% CI=1.263–3.779,2.190–6.921,0.251–0.681;all P<0.05).Conclusion:The up-regulation of vasohibin-1,MACC1 and down-regulation of KAI1 in serous ovarian cancer are related to the tumor differentiation,clinical stage,metastasis and prognosis.Combined detection of these indexes is useful in predicting the progr

关 键 词：浆液性卵巢癌 血管生成抑制蛋白-1 结肠癌转移相关蛋白1 抗癌1号蛋白 预后 

分 类 号：R737.31[医药卫生—肿瘤]