感觉神经肽与P物质缓释微球对小鼠拔牙窝成骨的作用  

Effects of Gelatin Microspheres Sustained Release CGRP and SP on Osteogensis of Tooth Socket of Mice

作　　者：宋子健 丁宇翔 周勤 SONG Zijian;DING Yuxiang;ZHOU Qin(State Key Laboratory of Military Stomatology&National Clinical Research Center for Oral Diseases&Shaanxi Clinical Research Center for Oral Diseases,Department of Oral Surgery,School of Stomatology,the Fourth Military Medical University,Xi'an 710032,China)

机构地区：[1]军事口腔医学国家重点实验室口腔疾病国家临床医学研究中心陕西省口腔疾病临床医学研究中心第四军医大学口腔医院颌面外科

出　　处：《口腔医学研究》2020年第1期20-23,共4页Journal of Oral Science Research

基　　金：国家自然科学基金(编号:81271103)

摘　　要：目的:验证负载感觉神经肽与P物质(substance P,SP)明胶缓释微球能否促进拔牙窝牙槽骨生成。方法:乳化交联法制备直径10~100μm的明胶微球,将10-6 mol/L的SP、神经肽降钙素基因相关肽(calcitonin gene-related peptide,CGRP)与微球混合制成载药缓释系统。27只成年KM小鼠随机分为SP组、CGRP组、SP/CGRP混合组,每组9只。分别拔除双侧上颌第一磨牙,一侧拔牙窝内放入含生理盐水的空白微球作为对照组,另一侧拔牙窝内放入载药微球作为实验组。于第4周处死小鼠,取上颌骨标本,制作硬组织切片,进行组织形态学分析,观察新骨形成情况。结果:术后4周测量新骨组织体积比例(bone volume/tissue volume,BV/TV),SP实验组为(30.25±0.92)%,同型对照组为(10.66±0.83)%,两组比较差异有统计学意义(P<0.05);CGRP实验组为(19.46±1.46)%,同型对照组为(8.78±1.03)%,两组比较差异有统计学意义(P<0.05);混合实验组为(59.45±3.07)%,同型对照组为(10.06±3.25)%,两组比较差异有统计学意义(P<0.05)。混合实验组为(59.453±3.071)%,混合空白组为(10.062±3.254)%,两组比较差异有统计学意义(P<0.05);骨小梁厚度(trabecular thickness,Tb.Th),混合空白组为(0.076±0.009)%,混合实验组为(0.132±0.043)%,两组比较差异有统计学意义(P<0.05);骨小梁数量(trabecular number,Tb.N),混合空白组为(0.841±0.212)%,混合实验组为(4.497±0.413)%,两组比较差异有统计学意义(P<0.05);骨密度(bone mineral density,BMD),混合空白组为(0.184±0.072)%,混合实验组为(0.615±0.010)%,两组比较差异有统计学意义(P<0.05)。结论:CGRP与SP均能够促进拔牙窝新骨生成,且两者对成骨具有协同促进作用。Objective:To explore whether the gelatin microspheres sustained release CGRP and SP can promote bone formation in tooth socket of mice.Methods:The gelatin microspheres containing 10-6 mol/L SP and CGRP were prepared and filled into the sockets of maxillary first molar on one side of 27 adult KM mice.The mice were divided into three groups,including SP,CGRP,and SP/CGRP mix groups randomly.4 weeks later,the mice were sacrificed and the maxillae bone was harvested.The samples were embedded in methylmethacrylate for histological morphology examination.Results:The average BV/TV values were(30.25±0.92)%in experimental socket and(10.66±0.83)%in control socket(P<0.05)in SP group;(19.46±1.46)%in experimental socket and(8.78±1.03)%in control socket(P<0.05)in CGRP group:(59.45±3.07)%in experimental socket and(10.06±3.25)%in control socket(P<0.05)in SP/CGRP mix group.Bone volume/tissue volume(BV/TV)was(59.453±3.071)%in the mixed experimental group and(10.062±3.254)%in the mixed blank group(P<0.05).Trabecular thicknesses(Tb.Th)were(0.076±0.009)%in the mixed blank group and 0.132±0.043%in the mixed treatment group(P<0.05).Trabecular number(Tb.N)were(0.841±0.212)%in the mixed blank group and(4.497±0.413)%in the mixed treatment group(P<0.05).Bone mineral density(BMD)was(0.184±0.072)%in mixed blank group was and(0.615±0.010)%in mixed experimental group(P<0.05).Conclusion:CGRP and SP could promote osteogenesis individually and promote osteogenesis synergistically.

关 键 词：感觉神经肽 P物质 明胶微球 牙槽骨 骨生成 

分 类 号：R782.11[医药卫生—口腔医学] R-332[医药卫生—临床医学]