姜黄素对慢性应激大鼠的神经保护作用  

Neuroprotective effect of curcumin on chronic unpredictable mild stress rats

作　　者：邓莉[1] 耿春梅[2] 廖德华[3] 江沛[2] 韩文秀 DENG Li;GENG Chunmei;LIAO Dehua;JIANG Pei;HAN Wenxiu(Department of Health Management,the First People′s Hospital of Jining City,Shandong Province,Jining 272002,China;Department of Clinical Pharmacy,the First People′s Hospital of Jining City,Shandong Province,Jining 272002,China;Department of Pharmacology,Hu′nan Cancer Hospital,Hu′nan Province,Changsha 410006,China)

机构地区：[1]山东省济宁市第一人民医院健康管理部,山东济宁272002 [2]山东省济宁市第一人民医院临床药学科,山东济宁272002 [3]湖南省肿瘤医院药学部,湖南长沙410006

出　　处：《中国医药导报》2019年第35期4-7,F0004,共5页China Medical Herald

基　　金：国家自然科学基金资助项目(81602846);泰山学者青年基金资助项目(tsqn201812159)

摘　　要：目的 探讨姜黄素对慢性应激引起的大鼠血清皮质酮水平、神经可塑性和神经凋亡的影响及其作用机制。方法 采用随机数字表法将24只SD大鼠分为对照组、模型组、治疗组,每组8只。模型组和治疗组给予慢性应激造模,治疗组给予100 mg/(kg·d)姜黄素,模型组及对照组给予相同体积的生理盐水。4周后,考察大鼠的行为学指标,测定血清皮质酮水平,海马BDNF、PSD-95、SYN蛋白表达及细胞凋亡情况。结果 模型组大鼠糖水偏好度显著低于对照组,治疗组大鼠糖水偏好度显著高于模型组(P <0.01);模型组大鼠不动时间显著长于对照组,治疗组大鼠不动时间显著短于模型组(P <0.01)。与对照组比较,模型组大鼠血清皮质酮水平显著升高(P <0.01);与模型组比较,治疗组大鼠血清皮质酮水平显著降低(P <0.01)。与对照组比较,模型组大鼠海马BDNF、PSD-95、SYN蛋白表达显著降低(P <0.05或P <0.01);与模型组比较,治疗组大鼠海马BDNF、PSD-95、SYN蛋白表达显著升高(P <0.05或P <0.01)。与对照组比较,模型组大鼠组织切片中凋亡阳性细胞个数明显增加,细胞凋亡指数显著升高(P <0.01);与模型组比较,治疗组大鼠组织切片中凋亡阳性细胞个数明显减少,细胞凋亡指数显著降低(P <0.01)。结论 本研究在抑郁大鼠模型中证明姜黄素的潜在抗抑郁作用,其作用机制可能与调节血清皮质酮水平、神经可塑性及抗凋亡作用有关。Objective To explore the effect and mechanism of curcumin on corticosterone level,neuroplasticity and apoptosis in chronic unpredictable mild stress(CUMS)rats.Methods Twenty-four SD rats were divided into control group,model group and treatment group by random number table method,with 8 rats in each group.Model group and treatment group were given chronic stress model,treatment group was given 100 mg/(kg·d)curcumin,model group and control group were given the same volume of normal saline.After 4 weeks,the behavioral indexes,serum corticosterone level,BDNF,PSD-95,SYN protein expression and apoptosis in hippocampus were measured.Results The sugar preference of model group was significantly lower than that of control group,and that of treatment group was significantly higher than that of model group(P<0.01).The immobility time of model group was significantly longer than that of control group,and that of treatment group was significantly shorter than that of model group(P<0.01).Compared with control group,the level of serum corticosterone in model group increased significantly(P<0.01);compared with model group,the level of serum corticosterone in treatment group decreased significantly(P<0.01).Compared with control group,the expression of BDNF,PSD-95 and SYN protein in the hippocampus of model group decreased significantly(P<0.05 or P<0.01);compared with model group,the expression of BDNF,PSD-95 and SYN protein in the hippocampus of treatment group increased significantly(P<0.05 or P<0.01).Compared with control group,the number of apoptotic positive cells and apoptotic index increased significantly in model group(P<0.01);compared with model group,the number of apoptotic positive cells and apoptotic index decreased significantly in treatment group(P<0.01).Conclusion The potential antidepressant effect of curcumin is first demonstrated in the rat model of depression.Its mechanism may be related to the regulation of serum corticosterone level,neural plasticity and anti apoptosis.

关 键 词：抑郁症 姜黄素 皮质酮 神经可塑性 细胞凋亡 

分 类 号：R-332[医药卫生]