高效液相色谱法同时测定糖足散中4种成分的含量  

Simultaneous determination of four constituents in Tangzu San by high performance liquid chromatography

作　　者：徐璐娜 夏苗芬[2] 周玉芳[3] 袁强[1] XU Lu′na;XIA Miaofen;ZHOU Yufang;YUAN Qiang(College of Pharmacy,Zhejiang Chinese Medical University,Zhejiang Province,Hangzhou310053,China;College of Traditional Chinese Medicine,Zhejiang Pharmaceutical College,Zhejiang Province,Ningbo315503,China;Department of Pharmacy,Zhejiang Hospital,Zhejiang Province,Hangzhou310013,China)

机构地区：[1]浙江中医药大学药学院,浙江杭州310053 [2]浙江医药高等专科学校中药学院,浙江宁波315503 [3]浙江医院药剂科,浙江杭州310013

出　　处：《中国医药导报》2020年第1期29-32,共4页China Medical Herald

基　　金：浙江省高职高专院校中青年专业带头人专业领军项目（lj2013011）;浙江省高等教育“十三五”第一批教学改革研究项目（jg2018 0547）

摘　　要：目的建立高效液相色谱法同时测定糖足散中4种成分的含量。方法采用SilverSil C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.1%磷酸为流动相,梯度洗脱,流速1.0 mL/min,柱温35℃,检测波长为240 nm,进样量10μL。结果羟基红花黄色素A、芍药苷、阿魏酸和β-蜕皮甾酮的回归方程分别为Y=12 183X-5645.7,Y=10 438X-22 481,Y=34 706X-1790.1,Y=13 500X-898.66,线性范围内线性关系良好,相关系数(r)均≥0.9995。平均加样回收率为96.86%~101.72%。结论该方法可为糖足散的质量控制研究提供科学依据。Objective To establish a high performance liquid chromatography method for the simultaneous content determination of four constituents in Tangzu San. Methods SilverSil C18(4.6 mm×250 mm, 5 μm) column was used as the column, acetonitrile-0.1% phosphoric acid was used as the mobile phase of the gradient elute, and the flow rate of 1.0 m L/min, the colomn temperature maintained at 35℃. With the detection wavelength of 278 nm. Results The regression equations of hydroxy safflower yellow A, paeoniflorin, ferulic acid and β-ecdysterone were Y = 12 183 X-5645.7, Y =10 438 X-22 481, Y = 34 706 X-1790.1, Y=13 500 X-898.66 respectively. The method showed good linearity(all r ≥0.9995) within the linear range. The average recoveries were in the range of 96.86%-101.72%. Conclusion The method can provide scientific basis for the quality control of Tangzu San.

关 键 词：糖足散 羟基红花黄色素A 芍药苷 阿魏酸 β-蜕皮甾酮 高效液相色谱法 含量测定 

分 类 号：R284.1[医药卫生—中药学]