面条矩阵任务行为学方法在脑缺血模型大鼠前肢运动功能评价中的应用  

Application of pasta matrix reaching task in rats for forelimb motor function test after stroke

作　　者：孙芳玲 刘敏 田欣 刘婷婷 单永治[3] 魏鹏虎 樊晓形 郭德玉 王文 赵国光[3] Sun Fangling;Liu Min;Tian Xin;Liu Tingting;Shan Yongzhi;Wei Penghu;Fan Xiaotong;GuoDeyu;Wang Wen;Zhao Guoguang(Department of Experimental Animal Laboratory,Xuanwu Hospital of Capital Medical University,Beijing 100053,China;Department of Experimental Animal Laboratory,Beijing Geriatric Medical Research Center,Beijing 100053,China;Department of Neurosurgery,Xuanwu Hospital of Capital Medical University,Beijing 100053,China)

机构地区：[1]首都医科大学宣武医院实验动物室,北京100053 [2]北京市老年病医疗研究所实验动物室,100053 [3]首都医科大学宣武医院神经外科,北京100053

出　　处：《中华行为医学与脑科学杂志》2019年第11期961-966,共6页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家科技部重点研发项目(2016YFC0103909);国家自然科学基金项目(81503049,81871009,81573633,81801288);北京市科委重点研发项目(Z161100000516008)。

摘　　要：目的构建科学的面条矩阵任务(pasta matrix reaching task,PMRT)行为学研究方法,综合评价脑损伤引起的感觉-运动功能障碍。方法制备面条矩阵任务装置,对21只SD大鼠进行14 d面条矩阵抓取训练,对经训练后能执行抓取任务的大鼠按照随机数字表法分为模型组(11只)和假手术组(6只)。模型组采用内皮≪-l(endothelin-l,ET-l)定点注射建立大鼠脑缺血模型,假手术组定点注射生理盐水。术后7 d开始,每天对大鼠患侧前肢抓取矩阵内面条的数量及位置进行记录。采用尼氏染色方法计算术后7 d、14 d、28 d脑梗死体积。结果术后7 d时,运动皮层、缺血导致模型组大鼠抓取面条数量由术前(33.43±1.02)根减少到(20.57±0.57)根,而且前肢所能到达位置也明显受限。术后28d时,模型组大鼠患肢抓取面条根数已增加到(26.85±0.98)根,但与假手术组[(32.33±1.45)根]相比差异仍有统计学意义(片3.198,P<0.05)o脑梗死体积由术后7 d时的(37.82±1,17)mm3缩小至术后28 d时的(24.35±0.38)mm3,也提示大鼠运动皮层损伤有一定程度的恢复(PvO.01)。结论面条矩阵抓取任务行为学评价方法能对脑卒中后前肢精细运动功能进行灵敏、可靠的综合评价,并解决卒中后康复治疗代偿性训练问题。Objective To construct a scientific behavioral research of pasta matrix reaching task(PMRT)and comprehensively evaluate sensory-motor dysfunction caused by brain injury.Methods Twenty-one SD rats were subjected to 14-days pasta matrix grasping training and then were randomly divided intomodel group(11 rats)and sham group(6 rats).Motor cortex ischemia was induced by injection of endothelin-1 in SD rats.The number of pasta grabed by the injured forelimb and the location in matrix were evaluateddaily 7 days after surgery.The infarct volume was measured by Nissl staining at the 7 days,14 days,and28 days after stroke.Results The number of pasta obtained by rats was reduced from(33.43±1.02)to(20.57±0.57)at 7 days post stroke in model group,and then increased to(26.85±0.98)at 28 days poststroke,although there was a significant difference between sham group(32.33±1.45)and ischemic group(￡=3.198,P<0.05).The frequency of retrieval from each slot of the pasta matrix represented that shamgroup demonstrated a significant gain in performance in the antero quadrant of the matrix compared to ischemicrats by the fourth week after stroke.The stroke volume was decreased from(37.82±1.17)mm3 at 7 days post-stroke to(24.35±0.38)mm3 at 28 days post-stroke,indicating brain recovery from ischemic injury.Conclusion The pasta matrix reaching task can function as a versatile and sensitive behavioral assay thatpermits experimenters to collect accurate outcome data and manipulate limb use to mimic human clinical phenomenaincluding compensatory strategies and focused rehabilitative training after stroke.

关 键 词：面条矩阵任务 运动功能 梗死体积 脑缺血 大鼠 

分 类 号：R74[医药卫生—神经病学与精神病学]