深海真菌Phomopsis tersa的次级代谢产物及其细胞毒活性研究  

Study on the secondary metabolites from the deep-sea fungus Phomopsis tersa and their cytotoxic activities

作　　者：陈闪冲 陈玉婵[2] 李赛妮 刘洪新[2] 刘昭明 李浩华[2] 郭珩 张捷[2] 章卫民[2] 朱爽 CHEN Shan-chong;CHEN Yu-chan;LI Sai-ni;LIU Hong-xin;LIU Zhao-ming;LI Hao-hua;GUO Heng;ZHANG Jie;ZHANG Wei-min;ZHU Shuang(School of Biosciences and Biopharmaceutics,Guangdong Pharmaceutical University,Guangzhou 510006,China;State Key Laboratory of Applied Microbiology Southern China,Guangdong Provincial Key Laboratory of Microbial Culture Collection and Application,Guangdong Open Laboratory of Applied Microbiology,Guangdong Institute of Microbiology,Guangzhou 510070,China)

机构地区：[1]广东药科大学生命科学与生物制药学院,广东广州510006 [2]广东省微生物研究所省部共建华南应用微生物国家重点实验室广东省菌种保藏与应用重点实验室广东省微生物应用新技术公共实验室,广东广州510070

出　　处：《中国海洋药物》2019年第6期8-14,共7页Chinese Journal of Marine Drugs

基　　金：广州市科技计划重点项目（21607020018）;广东省自然科学基金研究团队项目（2016A030312014）;广东省海洋经济创新发展区域示范专项项目（GD2012-D01-002）;广东省科学院建设国内一流研究机构行动专项资金项目（2019GDASYL-0103007）资助

摘　　要：目的研究深海真菌Phomopsis tersa FS441的次级代谢产物及其细胞毒活性。方法采用正、反相硅胶柱、凝胶柱和高效液相等多种色谱技术对菌株FS441的固体发酵产物进行分离纯化,并通过NMR、MS等波谱方法鉴定化合物结构,采用SRB法对得到的单体化合物进行细胞毒活性测试。结果从发酵物中分离纯化得到11个化合物,分别鉴定为:5α,8α-表二氧麦角甾-6,22-二烯-3β-醇(1)、麦角甾醇(2)、12-hydroxydehydrobotrydienol(3)、5,6-二羟基-2,3,6-三甲基环己-2-烯酮(4)、1,2-二羟基-3,4-脱氢蒽酮(5)、(1S,2S,3S,4R)-3-氯-4-(2-羟基丙吡啶-2-基)-1-甲基环己烷-1,2-二醇(6)、(-)-5-methylmellein(7)、酪醇(8)、胸腺嘧啶核苷(9)、(R)-(+)-甘油1-9(Z),12(Z)-十八碳二烯酸酯(10)、亚油酸(11)。结论化合物3~6为首次从Phomopsis属分离得到,化合物1和2对SF-268、MCF-7、HepG-2和A5494种肿瘤细胞株具有较弱的细胞毒活性。Objective To study the secondary metabolites of the deep-sea fungus Phomopsis tersa FS441 and their cytotoxic activities.Methods The compounds were separated and purified from the solid fermentation product of the strain FS441 by silica gel,reverse phase silica gel,Sephadex LH-20 and high performance liquid chromatography(HPLC),their structures were identified by NMR and MS spectroscopy.Compounds were tested for their cytotoxic activities by SRB method.Results Eleven compounds were isolated and purified from the fermentation product and identified as 5 a,8 a-epidioxyergosta-6,22-dien-3 b-ol(1),ergosterol(2),12-hydroxydehydrobotrydienol(3),5,6-dihydroxy-2,3,6-trimethylcyclohex-2-enone(4),l,2-dihydroxy-3,4-dehydromenthone(5),(1 S,2 S,3 S,4 R)-3-chloro-4-(2-hydroxypropan-2-yl)-1-methylcyclohexane-1,2-diol(6),(-)-5-methylmellein(7),tyrosol(8),thymidine(9),(R)-(+)-glycerol 1-9(Z),12(Z)-octadecadienate(10),linoleic acid(11).Conclusion Compounds 3~6 were isolated from the genus Phomopsis for the first time.Compounds 1 and 2 exhibited weak cytotoxic activities against SF-268,MCF-7,HepG-2 and A549 cell lines.

关 键 词：深海真菌 Phomopsis tersa 次级代谢产物 细胞毒活性 

分 类 号：R914[医药卫生—药物化学]