天麻通心脑方治疗动脉粥样硬化的实验研究及对血管内皮功能的影响  

Effect of Tianma Tongxinnao Formula on Experimental Atherosclerosis Rats and Effects of Vascular Endothelial Function

作　　者：李怀晶[1] 全碧波[1] 尚喜雨[1] LI Huaijing;QUAN Bibo;SHANG Xiyu(Department of Biochemistry and Molecular Biology,Nanyang Medical College,Nanyang 473061,Henan,China)

机构地区：[1]南阳医学高等专科学校生物化学与分子生物学教研室

出　　处：《中华中医药学刊》2019年第12期2999-3001,I0036,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：河南省科技厅一般项目(2015048278K)

摘　　要：目的:探讨天麻通心脑方治疗动脉粥样硬化大鼠的实验效果及对大鼠血管内皮功能的影响。方法:选取健康成年Wister雄性大鼠64只,采用随机数字表法分为正常组(普通饲料喂养)、高脂组(高脂饲料喂养)、对照组(普通饲料喂养+天麻通心脑方3.06 g/kg)、实验组(高脂饲料喂养+天麻通心脑方3.06 g/kg)各16只,连续喂养6周;对比各组大鼠的血清甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、血液流变学指标、血清一氧化氮(NO)、内皮素-1(ET-1)、肿瘤坏死因子-α(TNF-α)、白细胞介素-6(IL-6)的变化。结果:高脂组的血清TG、TC、LDL-C水平均高于正常组和对照组(P<0.05),高脂组的血清HLD-C低于正常组和对照组(P<0.05);实验组的血清TG、TC、LDL-C水平低于高脂组(P<0.05),实验组的血清HLD-C高于高脂组(P<0.05);高脂组的全血低切黏度、全血中切黏度、全血高切黏度、红细胞聚集指数均高于正常组和对照组(P<0.05);实验组的全血低切黏度、全血中切黏度、全血高切黏度、红细胞聚集指数低于高脂组(P<0.05);高脂组的ET-1、TNF-α、IL-6均高于正常组和对照组(P<0.05),高脂组的NO低于正常组和对照组(P<0.05);实验组的ET-1、TNF-α、IL-6低于高脂组(P<0.05),实验组的NO高于高脂组(P<0.05)。结论:天麻通心脑方治疗动脉粥样硬化大鼠具有显著的降血脂效果、同时减轻高血脂引起的血管内皮损伤、炎症反应、改善血液流变学指标。Objective:To explore the experimental effect of Tianma Tongxinnao Formula on atherosclerosis rats and its effect on vascular endothelial function in rats.Methods:A total of 64 healthy adult Wister male rats were randomly divided into normal group(normal feed feeding),high fat group(high fat diet)and control group(normal feed+Tianma Tongxinnao Formula 3.06 g/kg),16 rats in the experimental group(high-fat diet feeding+Tianma Tongxin Tongxinnao Formula 3.06 g/kg)were continuously fed for 6 weeks.The serum triglycerides(TG)and total cholesterol(TC)were compared between the groups.High-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),hemorheology,serum nitric oxide(NO),endothelin-1(ET-1),tumor necrosis factor-α(TNF-α)and interleukin-6(IL-6)changes were compared.Results:The serum levels of TG,TC and LDL-C in the high-fat group were higher than those in the normal group and the control group(P<0.05).The serum HLD-C in the high-fat group was lower than that in the normal group and the control group(P<0.05).The serum levels of TG,TC and LDL-C were lower than those in the high-fat group(P<0.05).The serum HLD-C of the experimental group was higher than that of the high fat group(P<0.05).The high-fat group’s blood viscosity,whole blood high-viscosity and erythrocyte aggregation index were higher than those in the normal group and the control group(P<0.05).The whole blood low-cut viscosity,whole blood cut viscosity,whole blood high-viscosity and red blood cells in the experimental group were lower than those in the high-fat group(P<0.05).The ET-1,TNF-αand IL-6 in the high fat group were higher than those in the normal group and the control group(P<0.05),and the NO in the high fat group was lower than normal.The ET-1,TNF-αand IL-6 levels in the experimental group were lower than those in the high fat group(P<0.05).The NO in the experimental group was higher than that in the high fat group(P<0.05).Conclusion:Tianma Tongxinnao Formula has significant hypolipidemic effect on treating atheros

关 键 词：天麻通心脑方 动脉粥样硬化 血管内皮功能 

分 类 号：R285.5[医药卫生—中药学]