小鼠胚胎移植技术优化及对巨细胞病毒净化研究  

Optimization of Mouse Embryo Transfer Technology and Purification of Cytomegalovirus

作　　者：杜凯 李文光[1] 吴德国 赵德明[2] DU Kai;LI Wenguang;WU Deguo;ZHAO Deming(Institute of Genetics and Developmental Biology Chinese Academy of Sciences,Beijing 100101,China;China Agriculture University,Beijing 100094,China)

机构地区：[1]中国科学院遗传与发育生物学研究所,北京100101 [2]中国农业大学,北京100094

出　　处：《实验动物科学》2019年第5期31-34,共4页Laboratory Animal Science

摘　　要：目的 建立小鼠巨细胞病毒净化方法,以获得无MCMV感染的小鼠。方法 利用胚胎移植技术,通过使用不同激素、不同发情周期注射激素、受体鼠品系、不同的移植方法、不同时期胚胎移植,以及移植胚胎数量等对比试验,优化了净化条件。利用优化的胚胎移植净化方法,对供体鼠进行了净化。结果 SIGMA生产的激素,在10 IU剂量下超排得到的可用胚胎数量约为17枚/只;在小鼠发情间期超排得到的可用胚胎最多,超排的可用胚胎数约为23枚/只;选取C57雄性小鼠与ICR雌性小鼠交配的子一代作为受体鼠,产仔率达44. 5%;输卵管移植较子宫移植效果好,产仔率为40. 64%;移植2细胞胚胎的妊娠率为80%,明显优于单细胞和8细胞;受体鼠移植24枚胚胎时,产仔率达到了43. 75%。利用优化的小鼠巨细胞病毒胚胎移植净化方法,净化得到了无MCMV感染的小鼠。结论 建立了小鼠巨细胞病毒净化方法,为获得无MCMV感染的小鼠种群提供了可靠的保障。Objective A mouse cytomegalovirus purification method was established to obtain mice without MCMV infection.Method The purification conditions were optimized by using different hormone,different estrous cycle injection hormone,receptor mouse strain,different transplantation method,different embryo transfer,and the number of transplanted embryos.The donor mice were purified by the optimized method of embryo transfer purification.Result The result showed that the number of embryos produced by SIGMA was over 17 at 10 IU.The number of embryos available for ovulatory superovulation is the largest,and the number of embryos available for superovulation is about 23.The offspring of C57 male mice and ICR female mice were the recipient m ice,with a litter size of 44.5%.The effect of tubal transplantation is better than that of uterus transplantation,with a birth rate of 40.64%.The pregnancy rate of transplanted 2 cell embryos was 80%,better than that of single cells and 8 cells.When the recipient mice transplanted 24 embryos,the litter size reached 43.75%.Mice without MCMV infection were purified by embryo transfer.Conclusion A purification method of murine cytomegalovirus was established to provide reliable protection for the population of mice without MCMV infection.

关 键 词：小鼠巨细胞病毒 胚胎移植 净化 

分 类 号：R-332[医药卫生]