UHRF1高表达对膀胱癌细胞Keap-1/Nrf2通路的调控作用分析  

Analysis of the Regulation of High Expression of UHRF1 on Keap-1/Nrf2 Pathway in Bladder Cancer Cells

作　　者：周昌东[1] 杨新平[1] 田玉新[1] 孙凯[1] 林洋[1] 张奇夫 ZHOU Changdong;YANG Xinping;TIAN Yuxin(Jilin Province Cancer Hospital,Changchun,130012)

机构地区：[1]吉林省肿瘤医院

出　　处：《实用癌症杂志》2020年第1期9-12,共4页The Practical Journal of Cancer

基　　金：吉林省直卫生专项项目(编号:sczsy201721)

摘　　要：目的探讨与分析泛素样含PHD和环指域1(UHRF1)高表达对膀胱癌细胞Keap-1/Nrf2通路的调控作用。方法分别用针对UHRF1的mimic及对照mimic转染膀胱癌细胞系UMUC3,标记为A组、B组,同时以PBS代替转染试剂的为C组。MTT检测细胞增殖,流式细胞术检测细胞凋亡,Transwell实验检测细胞侵袭,qRT-PCR检测mRNA表达,Western blot检测Keap-1/Nrf2通路蛋白表达。结果转染后48 h与72 h,与B组与C组相比,A组UHRF1 mRNA表达量显著增加(P<0.05)。转染后48 h与72 h,与B组与C组相比,A组的细胞增殖抑制率、凋亡率显著降低,细胞侵袭数目显著增加(P<0.05)。转染后48 h,与B组与C组相比,A组的Keap-1、Nrf2相对表达量显著增加(P<0.05)。结论UHRF1高表达能激活膀胱癌细胞Keap-1/Nrf2通路,从而促进细胞增殖与侵袭,抑制细胞凋亡。Objective To investigate and analyze the regulation of ubiquitin-like PHD and loop finger domain 1( UHRF1) expression on Keap-1/Nrf2 pathway in bladder cancer cells. Methods Bladder cancer cells line UMUC3 were transfected with UHRF1 mimic and control mimic and were labeled as group A and group B,and group C were replaced with PBS instead of transfection reagent. Cell proliferation were detected by MTT,apoptosis were detected by flow cytometry,cell invasion were detected by Transwell assay,mRNA expression were detected by qRT-PCR,and protein expression of Keap-1/Nrf2 pathway were detected by Western blot. Results Compared with group B and group C,the expression of UHRF1 mRNA in group A were significantly increased at 48 h and 72 h after transfection( P < 0. 05). At 48 h and 72 h after transfection,compared with group B and group C,the cell proliferation inhibition rates and apoptosis rates of group A were significantly decreased,and the number of cell invasion were significantly increased( P < 0. 05). At 48 h after transfection,the relative expression of Keap-1 and Nrf2 in group A were significantly increased compared with group B and group C( P < 0. 05). Conclusion High expression of UHRF1 can activate the Keap-1/Nrf2 pathway in bladder cancer cells,thereby promote cell proliferation and invasion and inhibiting apoptosis.

关 键 词：泛素样含PHD和环指域1 Keap-1/Nrf2通路 膀胱癌 细胞凋亡 细胞侵袭 

分 类 号：R737.14[医药卫生—肿瘤]