初级纤毛相关基因KIF3A通过调控EMT过程在口腔鳞状细胞癌中的作用机制研究  

Study on the mechanism of primary cilia-related gene KIF3A in oral squamous cell carcinoma by regulating EMT process

作　　者：玄云泽[1] 李书进 金成日[1] 杨康鹃 XUAN Yunze;LI Shujin;JIN Chengri;YANG Kangjuan(Department of Stomatology,Yanbian University Hospital,Yanji,Jilin 133000,China;Department of Cell Biology and Medical Genetics,School of Medicine,Yanbian University,Yanji,Jilin 133000,China)

机构地区：[1]延边大学附属医院口腔科,吉林延吉133000 [2]延边大学医学院细胞生物学与医学遗传学教研室,吉林延吉133000

出　　处：《重庆医学》2020年第1期7-12,共6页Chongqing medicine

基　　金：国家自然科学基金项目(81560179)

摘　　要：目的探讨驱动蛋白家族成员3A(KIF3A)在口腔鳞状细胞癌上皮间充质转化(EMT)过程中的作用及其调控机制。方法以人舌鳞癌细胞Tca8113作为研究对象分为KIF3A-siRNA转染组、阴性对照(NC)组、对照组。在荧光显微镜下观察Tca8113细胞的初级纤毛并计算其阳性检出率;采用Western blot和PCR检测各组KIF3A、Snail、E-钙黏蛋白、波形蛋白及mRNA的相对表达水平;Transwell实验观察Tca8113侵袭能力的变化;细胞划痕实验观察Tca8113迁移能力的变化。结果与对照组比较,KIF3A-siRNA转染组初级纤毛检出率明显降低(P<0.05);Western blot和PCR检测结果显示KIF3A-siRNA转染组KIF3A、Snail、波形蛋白及mRNA表达明显下调,而E-钙黏蛋白表达明显上调(P<0.05);细胞侵袭及划痕实验结果显示,与对照组比较,KIF3A-siRNA转染组Tca8113细胞侵袭、迁移能力明显降低(P<0.05)。结论沉默初级纤毛相关基因KIF3A可能通过调控Tca8113的EMT过程来抑制其侵袭、迁移能力。Objective To investigate the role and regulatory mechanism of KIF3A in the epithelial-mesenchymal transition(EMT)process of oral squamous cell carcinoma.Methods Human tongue squamous cell carcinoma cells Tca8113 were divided into the KIF3A-siRNA transfection group the negative control(NC)group and the control group.The primary cilia of Tca8113 cells were observed under the fluorescence microscope and the positive detection rate was calculated.Protein and mRNA levels of KIF3A,Snail,E-cadherin and Vimentin in each group were detected by Western blot and PCR.The invasive ability of Tca8113 cells was observed by Transwell.The migration ability of Tca8113 cells was observed by wound healing assay.Results Compared with the control group,the detection rate of primary cilia in the KIF3A-siRNA transfection group significantly reduced(P<0.05).The results of Western blot and qPCR showed that the protein and mRNA expressions of KIF3A,Snail and Vimentin in the KIF3A-siRNA transfection group significantly down-regulated,while the expression of E-cadherin significantly up-regulated(P<0.05).Compared with the control group,the cell invasion and migration ability of Tca8113 cells in the KIF3A-siRNA transfection group significantly reduced(P<0.05).Conclusion Silencing the primary cilia-related gene KIF3A may inhibit its invasion and migration ability by regulating the EMT process of Tca8113.

关 键 词：口腔肿瘤 癌 鳞状细胞 纤毛 上皮间充质转化 驱动蛋白家族成员3A 

分 类 号：R739.85[医药卫生—肿瘤]