四川蔬菜尾菜可培养乳酸菌多样性及优良菌株筛选  

Diversity of culturable lactic acid bacteria and screening of silage strains in vegetable waste from Sichuan province, China

作　　者：张俊涛 黄熙瀛 李蓉 向泉桔 张小平 辜运富 ZHANG Juntao;HUANG Xiying;LI Rong;XIANG Quanju;ZHANG Xiaoping;GU Yunfu(Department of Applied Microbiology,College of Resource Science and Technology,Sichuan Agricultural University,Chengdu 611130,China)

机构地区：[1]四川农业大学资源学院应用微生物学系

出　　处：《应用与环境生物学报》2019年第6期1428-1433,共6页Chinese Journal of Applied and Environmental Biology

基　　金：教育部春晖项目（Z2016116）资助~~

摘　　要：为了解四川地区蔬菜尾菜中乳酸菌(Lactic acid bacteria,LAB)的多样性,筛选适用于尾菜青贮发酵的高效乳酸菌菌株,运用纯培养法、16S rRNA-RFLP和系统发育分析对乳酸菌遗传多样性进行研究,并对分离菌株的产酸能力、耐酸能力、耐高温能力和抗菌能力等4个指标进行测定.结果显示,分离得到的120株乳酸菌中,有18株(占总分离菌株的15.0%)产酸能力较强,10株(8.3%)能耐受50℃高温,11株(9.2%)耐酸能力较强(pH=2.5),13株(10.8%)表现出较强的抗菌能力,而菌株C20(Lactobacillus plantarum)综合发酵特性最优.基于16S rRNA-RFLP聚类图选取10株代表菌株进行16S rRNA基因测序和系统发育分析,发现120株乳酸菌分属于明串珠菌属(Leuconostoc)、片球菌属(Pediococcus)、乳杆菌属(Lactobacillus)和肠球菌属(Enterococcus)4个属,其中明串珠菌属为优势菌.综上,四川地区蔬菜尾菜中蕴含着较为丰富多样的乳酸菌资源,这些乳酸菌在蔬菜尾菜的青贮饲料化利用方面表现出潜在价值.(图2表2参30)The aim of this study was to explore the diversity of lactic acid bacteria(LAB)present in vegetable waste samples from the Sichuan province,China.In this study,we screened potentially suitable isolates from vegetable waste silage and investigated the genetic diversity of the LAB isolates through bacterial culture,16S rRNA-RFLP,and 16S rRNA gene sequencing.We also evaluated the LAB isolates according to the following four indices:acid production,acid resistance,high-temperature resistance,and antibacterial activity.According to the test results,18 out of the 120 isolated LAB strains(15.0%of the total isolates)had strong acid-producing ability,10 strains(8.3%)were able to withstand temperatures of 50℃,11 strains(9.2%)could withstand an acidic environment(pH 2.5),and 13 strains(10.8%)showed strong antibacterial ability.The comprehensive fermentation characteristic of strain C20(Lactobacillus plantarum)was the best.According to the 16S rRNA-RFLP patterns and colony morphologies,ten typical strains were selected for 16S rRNA gene sequencing and phylogeny analyses.Phylogeny analyses showed that the strains were phylogenetically affiliated with the following groups:Leuconostoc,Pediococcus,Lactobacillus,and Enterococcus.Out of these groups,Leuconostoc bacteria were the predominant species.In conclusion,diverse species of LAB were present in vegetable waste samples from the Sichuan province,and they are potentially valuable for the silage of vegetable waste.

关 键 词：蔬菜尾菜 乳酸菌 多样性 分离筛选 16S rRNA 

分 类 号：S182[农业科学—农业基础科学]