光照及潮湿环境诱导的小鼠应激性抑郁模型的建立与评价  

Establishment and evaluation of a stress depression model induced by light and dampness on mice

作　　者：朱朕 欧阳雪岩 杨超 于瑞华 丁罡 吉永华[1,3] 姜峰 Zhu Zhen;Ouyang Xueyan;Yang Chao;Yu Ruihua;Ding Gang;Ji Yonghua;Jiang Feng(School of Life Sciences,Shanghai University,Shanghai 200444,China;Shanghai Jiao Tong University School of Medicine,Shanghai 200025,China;Chongming Branch,Xinhua Hospital Affiliated to Shanghai Jiao Tong University School of Medicine,Xinhua Translational Institute for Cancer Pain,Shanghai 202150,China;Shanghai International Medical Center,Shanghai 201318,China)

机构地区：[1]上海大学生命科学学院,200444 [2]上海交通大学医学院,200025 [3]上海交通大学医学院附属新华医院崇明分院,上海市崇明新华癌痛转化研究所,202150 [4]上海国际医学中心,201318

出　　处：《中华行为医学与脑科学杂志》2019年第12期1136-1140,共5页Chinese Journal of Behavioral Medicine and Brain Science

基　　金：国家自然科学基金项目(81572859);上海市卫生健康委员会科研课题(20184Y0016,201840003,201740027)。

摘　　要：目的通过光照时间改变和潮湿环境相结合的方法(Light-Dampness,L-D)建立一种小鼠应激性抑郁模型。方法选择体质量为18~21 g的C57BL/6的小鼠建立光照-潮湿(L-D)应激性抑郁模型。选取基线测试中旷场实验水平评分及垂直评分大于30分且小于120分的小鼠75只,实验分为造模实验和药物干预实验两部分。造模部分28只小鼠随机分为3组:对照组(9只)、CUMS模型组(10只)及光照-潮湿(L-D)模型组(9只);药物干预部分47只小鼠随机分为5组:对照组(9只)、CUMS模型组(10只)、L-D模型组(9只)、CUMS模型+氟西汀组(10只)、L-D模型+氟西汀组(9只)。采用旷场实验、强迫游泳实验和蔗糖偏好实验检测实验动物的抑郁状况。结果(1)造模后的行为学评价结果显示,旷场实验中,与对照组[(76.44±9.22)分]相比较,CUMS模型组[(67.20±5.81)分]和L-D模型组[(52.56±19.05)分]的水平评分均显著降低(t=2.645,t=3.387;均P<0.05),且L-D模型组水平评分较CUMS模型组低(t=2.321,P<0.05);强迫游泳实验中,与对照组[(39.67±21.12)s]相比,CUMS模型组[(60.90±10.34)s]与L-D模型组[(74.89±16.10)s]不动时间均显著延长(t=2.831,3.979;均P<0.05),且L-D模型组不动时间较CUMS模型组增加(t=2.278,P<0.05)。蔗糖偏好实验中,CUMS模型组[(72±7)%]与L-D模型组[(65±5)%]的蔗糖偏好百分比相较对照组[(81±12)%]均下降(t=2.195,3.875,均P<0.05)。L-D模型组蔗糖偏好率显著低于CUMS模型组(t=2.286,P<0.05)。(2)使用抗抑郁药物干预实验动物后,旷场实验中CUMS模型组[(65.60±6.43)分]和L-D模型组[(54.33±14.67)分]的水平评分同对照组[(75.78±8.27)分]相比均显著降低(t=3.011,3.861;均P<0.05),且L-D模型组较CUMS模型组低(t=2.235,P<0.05)。在垂直评分中,L-D模型组[(33.44±4.54)分]同对照组[(39.22±5.56)分]相比显著降低(t=2.553,P<0.05)。氟西汀干预CUMS模型和L-D模型水平评分和垂直评分较未干预组升高(t=3.090,2.692;均P<0.05,t=2.681,2.354;均P<0.05)。Objective To establish a novel stress-induced depression model by changing the lighting conditions and continuously damping cushion(L-D).Methods The L-D stress depression animal model was established in C57BL/6 mice with body weight of 18-22 g.Seventy-five mice with the horizontal and vertical scores higher than 30 and less than 120 in open field test were employed.In the research of model construction,mice were randomly divided into three groups:control group(n=9),chronic unpredictable mild stress(CUMS)model group(n=10)and L-D model group(n=9).In the drug intervention experiments,mice were randomly divided into five groups:control group(n=9),chronic unpredictable mild stress(CUMS)model group(n=10),L-D model group(n=9),CUMS+fluoxetine group(n=10)and L-D model+fluoxetine group(n=9).Open field test,forced swimming test and sucrose preference test were used to evaluate the degree of depression in animals.Results(1)In the open field test,the horizontal score of CUMS model group(67.20±5.81)and the L-D model group(52.56±19.05)were significantly lower than that of the control group(76.44±9.22)(t=2.645,t=3.387,both P<0.05).And horizontal score of the L-D model was significantly lower than that of the CUMS model(t=2.321,both P<0.05).In forced swimming experiment,compared with the control group((39.67±21.12)s),the immobility time of the CUMS model group((60.90±10.34)s)and the L-D model group((74.89±16.10)s)were significantly prolonged(t=2.831,3.979,both P<0.05).The immobility time of the L-D model group was also significantly higher than that of the CUMS model group(t=2.278,P<0.05).In the sucrose preference experiment,the percentage of sucrose preference in CUMS model group((72±7)%)and L-D model group((65±5)%)was lower than that in the control group((81±12)%)(t=2.195,3.875,both P<0.05).The percentage of sucrose preference of L-D model group was significantly higher than that of CUMS model group(t=2.286,P<0.05).(2)After intervention with antidepressants,the horizontal scores of the CUMS model group(65.60±6.43)and

关 键 词：应激性抑郁动物模型 潮湿垫料刺激 光照改变 小鼠 

分 类 号：R-332[医药卫生] R749.4