Simultaneous, hybrid single-molecule method by optical tweezers and fluorescence  

作　　者：Guoteng Ma Chunguang Hu Shuai Li Xiaoqin Gao Hongbin Li Xiaotang Hu 

机构地区：[1]State Key Laboratory of Precision Measuring Technology and Instruments,Tianjin University,300072,China [2]Nanchang Institute for Microtechnology of Tianjin University,Tianjin 300072,China [3]Department of Chemistry,University of British Columbia,Vancouver,BC V6T 1Z1,Canada

出　　处：《Nanotechnology and Precision Engineering》2019年第4期145-156,共12页纳米技术与精密工程（英文）

基　　金：supported by the National Key Research and Development Program of China [grant numbers 2016YFB1102203, 2017YFF0107003]

摘　　要：As studies on life sciences progress toward the single-molecule level,new experiments have put forward more requirements for simultaneously displaying the mechanical properties and conformational changes of biomolecules.Optical tweezers and fluorescence microscopy have been combined to solve this problem.The combination of instruments forms a new generation of hybrid single-molecule technology that breaks through the limitations of traditional biochemical analysis.Powerfulmanipulation and fluorescence visualization have beenwidely used,and these techniques provide new possibilities for studying complex biochemical reactions at the singlemolecule level.This paper explains the features of this combined technique,including the application characteristics of single-trap and dual-traps,the anti-bleaching method,and optical tweezers combined with epifluorescence,confocal fluorescence,total internal reflection fluorescence,and other fluorescence methods.Using typical experiments,we analyze technical solutions and explain the factors and principles that instrument designers should consider.This review aims to give an introduction to this novel fusion technology process and describe important biological results.As studies on life sciences progress toward the single-molecule level, new experiments have put forward more requirements for simultaneously displaying the mechanical properties and conformational changes of biomolecules. Optical tweezers and fluorescence microscopy have been combined to solve this problem. The combination of instruments forms a new generation of hybrid single-molecule technology that breaks through the limitations of traditional biochemical analysis. Powerful manipulation and fluorescence visualization have been widely used,and these techniques provide new possibilities for studying complex biochemical reactions at the singlemolecule level. This paper explains the features of this combined technique, including the application characteristics of single-trap and dual-traps, the anti-bleaching method, and optical tweezers combined with epifluorescence, confocal fluorescence, total internal reflection fluorescence, and other fluorescence methods.Using typical experiments, we analyze technical solutions and explain the factors and principles that instrument designers should consider. This review aims to give an introduction to this novel fusion technology process and describe important biological results.

关 键 词：SINGLE-MOLECULE Optical tweezers Confocal fluorescence FRET TIRF 

分 类 号：O62[理学—有机化学]