miR-23a通过调节PPP2R5E促进人舌鳞癌细胞的增殖  

MiR-23a Promoting Cell Proliferation of Human Tongue Squamous Cell Carcinoma Cell through Regulating PPP2R5E

作　　者：陶亚东 柳雪[2] 孙继红 霍峰[1] 郭洪杰[2] TAO Yadong(Affiliated Hospital of Chengde Medical University,Hebei Chengde 067000,China)

机构地区：[1]承德医学院附属医院口腔科,河北承德067000 [2]承德医学院校医院,河北承德067000

出　　处：《河北医学》2020年第1期88-93,共6页Hebei Medicine

基　　金：2016年承德市科学技术研究与发展计划项目,(编号:201606A043);河北省教育厅青年基金项目,(编号:QN2019079);承德医学院自然科学基金项目,(编号:201722)

摘　　要：目的: 探讨miR-23a在人舌鳞癌细胞增殖中的作用机制。 方法: 收集舌鳞癌的临床组织标本,并培养舌鳞癌细胞系Tca8113和CAL27。实时定量PCR检测miR-23a和PPP2R5E舌鳞癌组织中的表达。过表达或封闭miR-23a后,应用MTT、平板集落形成实验和生长曲线实验检测miR-23a和PPP2R5E对舌鳞癌细胞增殖的影响。荧光素酶报告实验验证miR-23a对PPP2R5E的调控关系。 结果: miR-23a在舌鳞癌组织中的表达明显上调(P<0.01),而且可以促进舌鳞癌细胞增殖。生物信息学预测和荧光素报告实验显示PPP2R5E是miR-23a的直接靶基因。PPP2R5E在舌鳞癌组织中表达降低,且抑制舌鳞癌细胞增殖。PPP2R5E过表达可以逆转miR-23a对舌鳞癌细胞的促进增殖作用。 结论: miR-23a可以通过PPP2R5E促进舌鳞癌细胞的增殖,在舌鳞癌的发生发展中发挥致癌基因功能。Objective: To investigate the effect mechanism of miR-23a on the proliferation of human tongue squamous cell carcinoma cells. Methods: Clinical tissue samples of tongue squamous cell carcinoma were collected and Tca8113 and CAL27 were cultured. Real-time quantitative PCR was performed to detect the expressions of miR-23a and PPP2R5E in Clinical tissue samples of tongue squamous cell carcinoma. MTT assay, colony formation assay and growth curve assay were used to detect the effect of miR-23a and PPP2R5E on the proliferation of human tongue squamous carcinoma cell. Luciferase reporter assay verified the regulatory relationship between mir-23a and PPP2R5E. Results: The expression of miR-23a in tongue squamous cell carcinoma was significantly up-regulated(P<0.01). MiR-23a promoted the proliferation of tongue squamous cell carcinoma cells. Bioinformatics prediction and luciferin reporting experiments showed that PPP2R5E was a direct target gene of miR-23a. The expression of PPP2R5E was decreased in tongue squamous cell carcinoma. PPP2R5E inhibited the proliferation of tongue squamous cell carcinoma cells. Overexpression of PPP2R5E can reverse the proliferation promoting effect of mir-23a on tongue squamous cell carcinoma cells. Conclusion: MiR-23a can promote the proliferation of tongue squamous cell carcinoma cells through PPP2R5E. MiR-23a plays an oncogene role in the occurrence and development of tongue squamous cell carcinoma.

关 键 词：舌鳞癌 miR-23a PPP2R5E基因 细胞增殖 

分 类 号：R739.86[医药卫生—肿瘤]