牛磺酸对急性氨中毒的鲫和草鱼抗氧化及炎症相关基因表达影响的比较  

Comparison effects of taurine on acute ammonia toxicity in genes involved in antioxidant and inflammation of Carassius auratus and Ctenopharyngodon idella

作　　者：章倩 张木子 黎明[1] 王日昕[1] ZHANG Qian;ZHANG Muzi;LI Ming;WANG Rixin(School of Marine Sciences,Ningbo University,Ningbo 315211,China)

机构地区：[1]宁波大学海洋学院

出　　处：《水产学报》2020年第2期289-299,共11页Journal of Fisheries of China

基　　金：国家自然科学基金(31872541,31872222)~~

摘　　要：为了比较牛磺酸对急性氨中毒的鲫和草鱼缓释作用的差异,实验分别构建了4个处理组,组1实验鱼通过腹腔注射生理盐水,组2注射醋酸铵(鲫7 mmol/g,草鱼9 mmol/g),组3注射醋酸铵和牛磺酸(100μg/g),组4注射牛磺酸。毒性实验持续96 h。结果显示,组2鲫肝脏中SOD、CuZnSOD和CAT基因mRNA表达量显著低于组1和组3;组2和组3鲫肝脏中GPx基因mRNA表达量显著低于组1;组1鲫大脑中SOD、CuZnSOD、CAT和GPx基因mRNA表达量最高;组2草鱼肝脏中SOD、CuZnSOD、CAT和GPx基因mRNA表达量显著高于其他组;组2和组4草鱼大脑中SOD和CuZnSOD基因mRNA表达量显著低于组1和组3;组3草鱼大脑中CAT和GPx基因mRNA表达量最高;此外,组2鲫和草鱼肝脏及大脑中TNF和IL基因mRNA表达量均显著高于其他组。研究表明,鱼类氨中毒会扰乱机体的抗氧化酶系统和免疫应答,引起氧化损伤和炎症反应;草鱼通过提高抗氧化相关基因表达以应对氨中毒;牛磺酸能够有效缓解氨中毒对鲫和草鱼造成的氧化伤害,但牛磺酸并不能降低氨中毒对鲫和草鱼造成的炎症反应。The four experimental groups were set up to test the response of Carassius auratus and Ctenopharyngodon idella to ammonia toxicity and taurine within 96 h.Group 1 was injected with NaCl,group 2 was injected with ammonium acetate(C.auratus 7 mmol/g;C.idella 9 mmol/g),group 3 was injected with ammonium acetate and taurine(100μg/g),and group 4 was injected with taurine.C.auratus in group 2 had lower mRNA expression of SOD,CuZnSOD and CAT in liver than those of tested fish in groups 1 and 3;tested fish in groups 2 and 3 had lower mRNA expression of GPx in liver than that in group 1;the highest mRNA expression of SOD,CuZnSOD,CAT and GPx in brain were found in group 1;C.idella in groups 2 had higher mRNA expression of SOD,CuZnSOD,CAT and GPx in liver;fish in groups 2 and 4 had lower mRNA expression of SOD and CuZnSOD in brain than those in groups 1 and 3;fish in group 3 had the highest mRNA expression of CAT and GPx in brain.C.auratus and C.idella in group 2 had the highest mRNA expression of TNF and IL in the brain and liver.This study indicates that defensive strategies are more effectively on C.idella in dealing with the ammonia challenge compared with C.auratus;the taurine could more effectively mitigate the adverse effect of oxidative stress on C.auratus and C.idella,but the inflammatory response in hyperammonemia C.auratus and C.idella was not alleviated by taurine.

关 键 词：鲫 草鱼 氨中毒 牛磺酸 氧化损伤 炎症反应 

分 类 号：Q786[生物学—分子生物学] S917.4[农业科学—水产科学]