乙烯利对青春期前SD雌性大鼠性早熟的影响及其机制研究  

Effect of ethephon on precocious puberty of prepubertal SD female rats

作　　者：徐莹 苟练 敬明武[2] 葛宇龙 刘科亮[2] 徐培渝 XU Ying;GOU Lian;JING Mingwu;GE Yulong;LIU Keliang;XU Peiyu(West China School of Public Health&West China Fourth Hospital,Sichuan University,Chengdu 610041;Sichuan Provincial Center for Disease Control and Prevention,Chengdu 610041,Sichuan,China)

机构地区：[1]四川大学华西公共卫生学院/华西第四医院,四川成都610041 [2]四川省疾病预防控制中心,四川成都610041

出　　处：《癌变．畸变．突变》2020年第1期39-42,共4页Carcinogenesis,Teratogenesis & Mutagenesis

摘　　要：目的:探讨乙烯利促进青春期前SD雌性大鼠性早熟的作用及相关分子机制。方法:先后两批SD雌性孕鼠自行产子后由亲鼠自养,雌仔鼠在出生15日龄(PND15)时,根据体质量,按窝别随机分为4组,分别为阴性对照组(蒸馏水)和低、中、高剂量(分别为134、269、538 mg/kg)乙烯利染毒组。各组动物均经口灌胃,每天1次,连续5 d。第1批动物用于观察阴道开口时间;第2批动物处死后取血液,下丘脑和垂体组织,后用酶联免疫吸附法(ELASA)检测血清FSH()促卵泡生成素和LH(黄体生成素)水平,实时荧光定量PCR(qPCR)检测下丘脑-垂体-性腺轴(HPGA)相关基因KISS-1、GPR54、GnRH、GnRHR的mRNA表达水平。结果:与阴性对照组相比,乙烯利中剂量组阴道开口时间提前(P<0.05);高剂量组阴道开口时间明显延长(P<0.05)。在qPCR检测结果中,与对照组相比,乙烯利中剂量组HPGA轴相关基因表达增加,高剂量组降低,且差异均有统计学意义(P<0.05)。而血清中LH、FSH水平的变化无统计学意义(P>0.05)。结论:中剂量时乙烯利具有促进青春期前SD大鼠性早熟的作用,其机制可能与影响HPGA轴相关基因表达有关。OBJECTIVE:To investigate the effect of ethephon and its mechanisms on precocious puberty of prepubertal SD female rats.METHODS:Postnatal day-15 SD female rats were randomly divided into 4 groups according to their body weights.Rats in the control group were given distilled water and the low,medium and high doses groups were given the ethephon at 134,269 and 538 mg/kg,respectively.Water and ethephon were given orally,once a day for 5 days.The first batch of animals was used to observe the time for vaginal opening.After the second batch of animals were killed,blood,hypothalamus and pituitary tissues were taken,and serum FSH and LH levels were detected by enzyme-linked immunosorbent assay(ELASA).Real-time quantitative PCR(qPCR)was performed to examine the mRNA expression levels of the hypothalamic-pituitary-gonadal axis(HPGA)-related genes KISS-1,GPR54,GnRH,and GnRHR.RESULTS:Compared with the control group,vaginal opening time of the ethephon middle dose group was advanced(P<0.05)and that for the high dose group was significantly prolonged(P<0.05).In addition,qPCR analyses show that expression of HPGA axis-related genes in the ethephon middle dose group was increased,and the high-dose group was decreased,and the difference was statistically significant(P<0.05).There was no significant difference in serum LH and FSH levels(P>0.05).CONCLUSION:Ethephon exposure was effective in promoting precocious puberty in prepubertal SD rats at the medium dose,and its mechanism may be related to the expression of genes related to the HPGA axis.

关 键 词：乙烯利 青春期前雌鼠 性早熟 下丘脑-垂体-性腺轴 

分 类 号：R394.6[医药卫生—医学遗传学]