甲状腺癌中miR-222关键靶基因预测及其信号通路分析  

Prediction of Key Target Genes of miR-222 in Thyroid Cancer and Analysis of Its Signal Pathway

作　　者：黄琪峰 郑琳琳 张菁[1] HUANG Qi-feng;ZHENG Lin-lin;ZHANG Jing(Department of Pharmacy,Run Run Shaw Hospital,Zhejiang University School of Medicine,Hangzhou 310016,Zhejiang,China;Department of Ultrasound,Run Run Shaw Hospital,Zhejiang University School of Medicine,Hangzhou 310016,Zhejiang,China)

机构地区：[1]浙江大学医学院附属邵逸夫医院药学部,浙江杭州310016 [2]浙江大学医学院附属邵逸夫医院超声科,浙江杭州310016

出　　处：《医学信息》2020年第1期85-88,共4页Journal of Medical Information

基　　金：浙江省自然科学基金/药学会联合基金(编号：LYY18H310004)

摘　　要：目的利用生物信息学技术预测人类miR-222的靶基因,并分析其可能参与甲状腺癌的生物学过程和信号通路。方法通过TCGA数据库研究miR-222的表达与甲状腺癌的关系,然后TCGA中表达下调的基因和网站预测的miR-222靶基因进行取交集得到重叠基因,再进行生物信息学分析确定与甲状腺癌相关的关键miR-222靶基因和通路,最后通过GEPIA对靶基因进行表达验证以及miR-222和靶基因的相关性分析。结果miR-222在甲状腺癌中表达上调,富集分析表明miR-222很有可能参与血管紧张素受体-配体结合的调节,其中一个可能的靶基因是AGTR1,其在甲状腺癌中表达下调,与miR-222的表达呈负相关。结论上调的miR-222可能以AGTR1为靶标,从而调节甲状腺癌中血管紧张素受体-配体结合发挥作用。Objective To predict the target genes of human miR-222 using bioinformatics technology,and analyze the biological processes and signal pathways that miR-222 may participate in.Methods TCGA database was used to study the relationship between miR-222 expression and thyroid cancer,then the genes with down-regulated expression in TCGA and miR-222 target genes predicted by the website were intersected to obtain overlapping genes,and bioinformatics analysis was performed to determine the correlation with thyroid cancer the key miR-222 target genes and pathways,and finally the target genes were verified by GEPIA and the correlation analysis between miR-222 and target genes.Results miR-222 expression was significantly up-regulated in thyroid cancer.Enrichment analysis showed that miR-222 is likely to be involved in the regulation of angiotensin receptor-ligand binding.One of the possible target genes is AGTR1,which is expressed in thyroid cancer.Down-regulation was significantly negatively correlated with miR-222 expression.Conclusion The up-regulated miR-222 is likely to target AGTR1,which may play a role in regulating angiotensin receptor-ligand binding in thyroid cancer.

关 键 词：甲状腺癌 MIR-222 AGTR1 生物信息学 

分 类 号：R736.1[医药卫生—肿瘤]