LDB2在肺癌组织中的表达及与S1PR1的相关性分析  

Expression of LDB2 in lung cancer tissues and its correlation with S1PR1

作　　者：褚福营[1] 王子杰 刘红利[3] 王塑 曹兴建[1] 陈相[1] CHU Fuying;WANG Zijie;LIU Hongli;WANG Su;CAO Xingjian;CHEN Xiang(Department of Laboratory Medicine,Nantong First People′s Hospital,Nantong 226001,Jiangsu;School of Xinglin,Nantong University,Nantong 226001,Jiangsu;Department of Laboratory Medicine,Nantong Tumor Hospital,Nantong 226001,Jiangsu,China)

机构地区：[1]南通市第一人民医院检验科,江苏南通226001 [2]南通大学杏林学院,江苏南通226001 [3]南通市肿瘤医院检验科,江苏南通226001

出　　处：《临床检验杂志》2019年第12期911-914,922,共5页Chinese Journal of Clinical Laboratory Science

基　　金：南通市卫生和计划生育委员会项目(wkzl2018003);南通市科技局项目(YYZ17010);南通市卫生局项目(WQ2014010)

摘　　要：目的探讨LIM域结合蛋白2(LDB2)在肺癌组织中的表达及其与1-磷酸神经鞘氨醇受体1(S1PR1)的相关性。方法选取2010年4月至2011年5月南通市肿瘤医院就诊的52例肺癌患者的癌组织作为实验组,相应的癌旁组织作为对照组。采用实时荧光定量PCR(qRT-PCR)检测两组LDB2及S1PR1基因的表达水平;通过Oncomine数据库对LDB2的表达结果进行验证,并分析其与临床病理参数之间的关系;绘制ROC曲线,评价其对肺癌的诊断价值;利用Kaplan-Meier Plotter数据库分析其与肺癌预后的关系;同时检测其与S1PR1的相关性。结果LDB2基因在肺癌组织中的表达量为0.158(0.062,0.383),癌旁组织中表达量为0.403(0.261,0.711),两组间差异具有统计学意义(U=700.0,P<0.01)。Oncomine数据库共检索出9项符合筛选条件的研究,其LDB2基因均呈低表达(P<0.01)。肺癌组织中LDB2基因的表达与性别、年龄、吸烟情况、病理类型、肿瘤大小、TNM分期及是否淋巴结转移均无关(P>0.05)。ROC曲线分析结果表明,当ROC曲线下面积(AUC ROC)为0.741(95%CI:0.643~0.839),cut-off值取0.247时,LDB2筛查肺癌的敏感性为80.8%,特异性为61.5%。Kaplan-Meier生存分析发现,低表达LDB2患者的5年总生存期显著低于高表达患者(P<0.01)。S1PR1基因在肺癌组织中表达量为0.710(0.337,1.523),在癌旁组织中的表达量为1.582(0.913,3.533),两组间差异具有统计学意义(U=780.0,P<0.01),且肺癌组织中LDB2与S1PR1的表达水平呈正相关(r=0.827,P<0.01)。结论LDB2及S1PR1在肺癌中均低表达且呈强相关性,两者在肺癌的发生、发展中具有重要的作用。Objective To investigate the expression of LIM domain binding 2(LDB2)in lung cancer tissues and its correlation with sphingosine-1 phosphate receptor 1(S1PR1).Methods Lung cancer tissues and the corresponding adjacent tissues from 52 patients in Nantong Tumor Hospital during April 2010 and May 2011 were collected as the experimental group and the control group,respectively.The expression levels of LDB2 and S1PR1 were detected by the real-time PCR(qRT-PCR).The expression results of LDB2 gene were further verified by the Oncomine database,and its correlations with clinicopathological parameters were analyzed.The ROC curve was drawn to evaluate the diagnosis value of LDB2 expression in lung cancer.The correlation of LDB2 expression with the prognosis of lung cancer was analyzed by the“Kaplan-Meier Plotter”database.In addition,the relationship between LDB2 and S1PR1 was also analyzed.Results The expression levels of LDB2 in lung cancer tissues(0.158[0.062,0.383])were significantly lower than that in the adjacent tissues(0.403[0.261,0.711],U=700.0,P<0.01).A total of 9 eligible studies were retrieved from the Oncomine database,and their expressions of LDB2 were also low(P<0.01).The expressions of LDB2 in lung cancer tissues were not related to gender,age,smoking history,pathological type,tumor size,TNM staging and lymphatic metastasis(P>0.05).The results of ROC curve showed that when the area under the ROC curve(AUC ROC)was 0.741(95%CI:0.643-0.839)and the cut-off value was 0.247,the sensitivity and specificity of LDB2 in the diagnosis of lung cancer were 80.8%and 61.5%,respectively.The Kaplan-Meier survival analysis showed that the 5-year overall survival time of the patients with low expression of LDB2 was shorter than that of the patients with high expression of LDB2(P<0.01).In addition,the expression levels of S1PR1 in lung cancer tissues(0.710[0.337,1.523])were significantly lower than that in the adjacent tissues(1.582[0.913,3.533],U=780.0,P<0.01),and the expression levels of S1PR1 in lung cancer tissues were

关 键 词：LIM域结合蛋白2 1-磷酸神经鞘氨醇受体1 肺癌 相关性 

分 类 号：R734.2[医药卫生—肿瘤]