白鲜碱对气管平滑肌的舒张作用及机理研究  

Relaxation effect and mechanism of dictamnine on tracheal smooth muscle

作　　者：沈金花 韩淑慧 时顺波 SHEN Jinhua;HAN Shuhui;SHI Shunbo(Institute for Medical Biology&Hubei Provincial Key Laboratory for Protection and Application of Special Plant Germplasmin Wuling Area of China,College of Life Sciences,South⁃Central University for Nationalities,Wuhan 430074,China)

机构地区：[1]中南民族大学生命科学学院医学生物研究所&武陵山区特色资源植物种质保护与利用湖北省重点实验室

出　　处：《中南民族大学学报（自然科学版）》2020年第1期24-28,共5页Journal of South-Central University for Nationalities：Natural Science Edition

基　　金：国家自然科学基金资助项目(31771274)

摘　　要：目的:探讨白鲜碱对异常收缩的气管平滑肌的舒张作用.方法:以80 mmol·L^-1 KCl或100μmol·L^-1 ACh预刺激气管,用生物机能系统检测气管的张力,用膜片钳技术检测离子通道电流,观察白鲜碱对气管平滑肌的影响.结果:白鲜碱能呈剂量依赖性地抑制高钾和ACh诱导的预收缩,而对静息状态下的气管无影响.且白鲜碱能阻断VDCCs电流,抑制细胞外Ca^2+内流.除VDCCs通道外,它还抑制ACh激活的其他离子通道,并抑制肌质网中的内Ca^2+释放.结论:白鲜碱具有舒张气管平滑肌异常收缩的作用,有望作为一种钙离子拮抗剂抑制气道高反应,缓解哮喘.Objective: To investigate the relaxation of dictamnine on abnormally contracted tracheal smooth muscle. Methods: The trachea was prestimulated with 80 mmol·L^-1 KCl or 100 μmol·L^-1 ACh, the tracheal tension was measured by using a biometric system, and the ion channel currents were examined by patch clamp technique. The effect of dictamnine on tracheal smooth muscle was then observed. Results: Dictamnine inhibited the hyperkalemia and ACh-induced precontraction in a dose-dependent manner, but had no effect on the trachea at rest. Dictamnine blocked VDCCs current and inhibited extracellular Ca^2+ influx. In addition to the VDCCs channel, dictamnine also inhibited other ion channels activated by ACh and inhibited the release of Ca^2+ in the sarcoplasmic reticulum. Conclusion: Dictamnine has the effect of relaxing the contraction of tracheal smooth muscle and is expected to act as a calcium channel blockers to inhibit AHR and relieve asthma symptom.

关 键 词：白鲜碱 气管平滑肌 钙离子 哮喘 

分 类 号：Q474[生物学—生理学] R734.2[医药卫生—肿瘤]