金川雪梨和秦酥挥发性芳香物质的SPME-GC-MS分析  

Analysis of SPME-GC-MS on Volatile Aromatic Compounds of Jinchuanxue Pear and Qinsu

作　　者：王颖[1] 张文君 李慧冬 丁蕊艳 方丽萍 郭长英 毛江胜 杨亲正[1] 陈子雷 WANG Ying;ZHANG Wen-jun;LI Hui-dong;DING Rui-yan;FANG Li-ping;GUO Chang-ying;MAO Jiang-sheng;YANG Qin-zheng;CHEN Zi-lei(School of Bioengineering,Qilu University of Technology,Shandong Academy of Sciences,Jinan 250353,Shandong,China;Institute of Agricultural Quality Standards and Testing Technology,Shandong Academy of Agricultural Sciences,Jinan 250100,Shandong,China;College of Life Sciences,Shandong Normal University,Jinan 250014,Shandong,China)

机构地区：[1]齐鲁工业大学(山东省科学院)生物工程学院,山东济南250353 [2]山东省农业科学院农业质量标准与检测技术研究所,山东济南250100 [3]山东师范大学生命科学学院,山东济南250014

出　　处：《食品研究与开发》2020年第1期160-166,共7页Food Research and Development

基　　金：国家现代梨产业技术体系专项（CARS-28-23）;农业检验监测公共服务平台（CXGC2017A03）

摘　　要：梨果香气成分分析对于梨果的香气育种、采收、病害防治以及贮藏加工有着重要意义。选取金川雪梨和秦酥两种典型白梨,通过对萃取头种类、萃取温度和时间等萃取条件优化、方法可行性验证,建立挥发性香气物质的固相微萃取-气相色谱质谱联用(solid phase microextraction-gas chromatography-mass spectrometry,SPME-GC-MS)分析方法。结果显示:各物质在5 mg/L^200 mg/L的范围内线性良好,相关系数R2均大于0.99,各物质的最小检出限小于2.44×10-3ng;添加回收试验中,各标准物质在梨块中添加回收率为89.3%~101.4%,相对标准偏差为1.6%~7.9%,满足香气物质分析要求;金川雪梨可检测出挥发性芳香物质达63种,秦酥挥发性芳香物质达46种。通过香气值计算,确定两种梨果中可定量特征香气,种类及数量因品种不同而不同。Analysis of aroma components of pear fruits was of great significance for pear fruits aromatic bred,harvested,disease prevented and stored,processed. Two typical white pears of Jinchuanxue pear and Qinsu were selected,through optimized the extraction conditions such as the type of extraction head,extraction temperature and time, and feasibility verification of the method, solid phase microextraction-gas chromatography-mass spectrometry(SPME-GC-MS)analysis method was established for the formation of volatile aroma substances. The results showed that:Each substance had a well linearity in the range of 5 mg/L to200 mg/L,the correlation coefficient R2 was greater than 0.99,the minimum detection limit of each substance was less than 2.44×10-3 ng;In the addition recovery test,the recovery of each standard substance in the pear block was 89.3 % to 101.4 %,and the relative standard deviation was 1.6 % to 7.9 %,which satisfied the analysis of aroma substances;There were 63 volatile aromatic compounds could detected from Jinchuanxue pear and 46 volatile aromatic compounds could detected from Qinsu. Through the calculation of the aroma value,the quantitative characteristic aroma of the two kinds of pear fruits was determined,and the types and quantities varied with the varieties.

关 键 词：金川雪梨 秦酥 香气成分 定性定量分析 固相微萃取-气相色谱质谱联用 

分 类 号：O657.63[理学—分析化学] TS255.7[理学—化学]