基于钙离子转运调控的甘草酸抗过敏作用  

Anti-Allergic Mechanism of Glycyrrhizic Acid Based on Calcium Transport Regulation

作　　者：姜天依 孙璐[1] 普秋榕 张亚妮 车会莲[1] JIANG Tianyi;SUN Lu;PU Qiurong;ZHANG Yani;CHE Huilian(Beijing Advanced Innovation Center for Food Nutrition and Human Health,College of Food Science&Nutrition Engineering,China Agricultural University,Beijing 100083,China)

机构地区：[1]北京食品营养与人类健康高精尖创新中心中国农业大学食品科学与营养工程学院

出　　处：《食品科学》2020年第1期127-132,共6页Food Science

基　　金：“十三五”国家重点研发计划重点专项（2016ZX08011006-003）

摘　　要：为探明甘草酸(glycyrrhizic acid,GA)抗过敏机制、深入开发甘草保健功能,本研究利用雌性BALB/c小鼠致敏模型、被动皮肤过敏反应模型、过敏性皮炎模型和大鼠嗜碱性粒细胞RBL-2H3,系统分析GA的抗过敏作用,并初步研究其机制。结果显示:GA能有效抑制被动皮肤过敏小鼠的血管通透性和血清组胺水平。酶联免疫吸附测定分析发现,10 mg/kg mb或更高剂量的GA灌胃处理极显著降低了过敏性皮炎小鼠血清中肿瘤坏死因子α水平(P<0.01)。采用RBL-2H3细胞进行研究发现,GA能显著降低细胞β-氨基己糖苷酶的释放率(P<0.05),表明GA对肥大细胞脱颗粒有抑制作用。钙离子荧光探针Fluo-3 AM的分析结果显示,GA能抑制抗原诱导的胞内Ca2+水平上升。实时荧光定量聚合酶链式反应分析和免疫印迹实验结果显示,GA显著降低了钙离子通道调控蛋白STIM1和TRPC1的转录和表达水平。综上,GA可能通过调控钙离子释放和转运蛋白因子的表达水平、降低抗原激发后的胞内钙离子浓度,进而发挥其抑制肥大细胞脱颗粒的抗过敏作用。This study aimed to explore the effects of glycyrrhizic acid(GA) on allergic reactions by using sensitized BALB/c female mice model, passive cutaneous anaphylaxis reaction and atopic dermatitis model and rat basophil leukemia RBL-2 H3 cells and to explore the underlying mechanism. The results showed that GA could effectively inhibit vascular permeability and histamine level in serum of passive skin allergic mice. The serum tumor necrosis factor receptor-α level in mice with allergic dermatitis, as determined by enzyme-linked immunosorbent assay, was significantly decreased after GA administration at 10 mg/kg mb or above. Experiments with RBL-2 H3 cells showed that GA could significantly reduce the release rate of β-hexosaminidase, thereby inhibiting the degranulation of mast cells. The fluorescent calcium probe Fluo-3 AM was used to analyze RBL-2 H3 cells sensitized by IgE. It turned out that GA could inhibit the increase of intracellular Ca2+ level induced by antigen. Quantitative real-time polymerase chain reaction and Western blot analysis showed that GA significantly decreased the mRNA transcription and protein expression of calcium channel regulatory proteins STIM1 and TRPC1. These results suggest that GA can inhibit degranulation by regulating the expression of calcium release and transporter factors and decreasing intracellular calcium concentration after antigen stimulation.

关 键 词：甘草酸 抗过敏活性 被动皮肤过敏反应 

分 类 号：R392.8[医药卫生—免疫学] TS253[医药卫生—基础医学]