TFB1M在结直肠癌中的表达及意义  

Expression and significance of TFB1M in colorectal cancer

作　　者：杨世荣 陈艳琴 白翔宇 甘露 何显力 YANG Shirong;CHEN Yanqin;BAI Xiangyu;GAN Lu;HE Xianli(Department of General Surgery,Tangdu Hospital,Air Force Medical University,Xi'an 710032,China;Department of Clinical Medicine,Medical College of Yan'an University,Yan'an 716000,China;Pharmaceutical College of Henan University,Kaifeng 475004,China;Department of Orthopedics,Air Force Medical Center,Beijing 100142,China;PLA 94647 Troops Hospital,Fuzhou 350026,China)

机构地区：[1]空军军医大学唐都医院普外科,西安710032 [2]延安大学医学院临床医学系,延安716000 [3]河南大学药学院,开封475004 [4]空军特色医学中心骨科,北京100142 [5]中国人民解放军94647部队医院,福州350026

出　　处：《中国癌症防治杂志》2019年第6期492-497,共6页CHINESE JOURNAL OF ONCOLOGY PREVENTION AND TREATMENT

基　　金：国家国际科技合作专项项目（2013DFA32110）

摘　　要：目的探讨线粒体转录因子B1(mitochondrial transcription factor B1,TFB1M)在结直肠癌组织中的表达及其意义。方法收集112例结直肠癌患者手术切除样本及临床信息,采用免疫组化法检测结直肠癌组织及相应癌旁正常组织中TFB1M的表达,并下载癌症基因组图谱(the Cancer Genome Atlas,TCGA)公共数据库中结直肠癌患者转录组数据及临床信息,分析TFB1M表达与患者临床病理特征及预后的关系。在结直肠癌SW620和HCT116细胞株中干涉及过表达TFB1M后,采用MTS检测细胞增殖能力。结果TFB1M在结直肠癌组织中阳性表达评分显著低于癌旁正常组织[(3.79±1.66)分vs(8.14±1.82)分,P<0.01]。TCGA数据和临床样本信息分析结果显示,TFB1M在Ⅲ+Ⅳ期患者中的表达量均较Ⅰ+Ⅱ期患者显著降低(P<0.01),TFB1M高表达的结直肠癌患者总生存期均优于低表达患者(P<0.05),且TFB1M是影响结直肠癌患者总生存期的独立因素。干涉TFB1M表达后,结直肠癌SW620和HCT116细胞增殖能力均较对照组显著增强(P<0.001);而过表达TFB1M细胞增殖能力均下降P<0.001。结论TFB1M低表达患者预后较差,干涉TFB1M可促进结直肠癌细胞增殖,TFB1M可能作为结直肠癌患者预后预测的生物标志物。Objective To investigate the expression mitochondrial transcription factor B1(TFB1M)in colorectal cancer tissue and its significance.Methods A total of 112 cases of surgical resection of patients with colorectal cancer and their clinical information were collected,and the expression level of TFB1M in colorectal cancer tissues and paired adjacent normal tissues were detected by Immunohistochemistry.The transcriptome data and clinical information were downloaded from the Cancer Genome Atlas(TCGA)database,and the relationship between the expression of TFB1M and clinical pathological characteristics and prognosis of patients with colorectal cancer.The cell proliferation was determined by MTS assay after interfering and overexpressing TFB1M in SW620 and HCT116 cells.Results The positive expression score of TFB1M in colorectal cancer tissues was significantly lower than that in adjacent normal tissues[(3.79±1.66)points vs(8.14±1.82)points,P<0.01]TFB1M expression in stageⅢ+Ⅳpatients with colorectal cancer were significantly lower than stageⅠ+Ⅱpatients through analyzing TCGA database and clinical information.Furthermore,the overall survival of patients with high expression of TFB1M was better than that of patients with low expression(P<0.01),and TFB1M was an independent factor affecting the overall survival of patients with colorectal cancer. After interfering with TFB1M expression,the proliferation of colorectal cancer SW620 and HCT116 cells were significantly enhanced compared with the control group,while the proliferation of overexpressing TFB1M cells was decreased. Conclusions Low TFB1M expression has a poor prognosis. Interfering with TFB1M can promote colorectal cancer cell proliferation. TFB1M may be used as a biomarker for predicting the prognosis of colorectal cancer patients.

关 键 词：结直肠癌 TFB1M 癌症基因组图谱 表达 预后 

分 类 号：R735.35[医药卫生—肿瘤] R735.37[医药卫生—临床医学]