微RNA-346对食管癌细胞生物学行为影响的分子机制研究  

Molecular mechanism of miR-346 on the biological behavior of esophageal cancer cells

作　　者：赵明理[1] 刘雷 曹建西[1] 崔琳[1] ZHAO Mingli;LIU Lei;CAO Jianxi;CUI Lin(Department of Cardiothoracic Surgery,the First Affiliated Hospital of Henan University of Traditional Chinese Medicine,Zhengzhou,Henan 450000,China)

机构地区：[1]河南中医药大学第一附属医院心胸外科

出　　处：《安徽医药》2020年第2期259-262,共4页Anhui Medical and Pharmaceutical Journal

基　　金：河南省中医药科学研究专项课题(2019ZY1203);河南省高等学校重点科目项目(20B360006)

摘　　要：目的观察微RNA-346(miR-346)对食管癌细胞生物学行为的影响,并探讨其分子机制。方法通过实时荧光定量PCR(q PCR)检测食管癌组织和细胞株miR-346的表达量。以表达量最低的细胞为感染对象,分别感染miR-346慢病毒(实验组)和空载慢病毒(对照组)。MTT法和集落形成实验分别检测细胞活力和增殖能力,流式细胞术检测凋亡率。qPCR和蛋白质印迹法检测细胞中Yes相关蛋白1(YAP1)及细胞周期蛋白E(Cyclin E)、细胞凋亡抑制蛋白1(cIAPl)表达水平。结果食管癌组织和细胞株中miR-346表达量均明显下降(P<0.01),TE-1细胞下降最明显。实验组感染miR-346的细胞活力(实验组0.93±0.06,对照组1.37±0.06)和增殖能力(对照组232.3±37.40,实验组82.55±9.66)降低(P<0.01),细胞凋亡率升高(实验组8.26%±0.80%,对照组0.94%±0.28,P<0.01),YAP1、Cyclin E和cIAPl蛋白表达显著下调。结论 miR-346在食管癌组织和细胞中低表达,可明显抑制TE-1细胞的增殖和诱导凋亡,其作用机制可能是靶向干扰YAP1基因的表达。Objective To observe the effect of microRNA-346(miR-346)on the biological behavior of esophageal cancer cells and discuss its molecular mechanism.Methods The expression of miR-346 in esophageal cancer tissues and cell lines was detected by real-time quantitative PCR(qPCR).The cells with the lowest expression of miR-346 were infected with miR-346 lentivirus(experimental group)and empty lentivirus(control group).MTT assay and colony formation assay were used to detect the viability and proliferation of cells.Flow cytometry was used to detect the apoptotic rate.The expressions of Yes related protein 1(YAP1),cyclin E and apoptosis protein 1(cIAPl)were detected by qPCR and Western blot.Results The expression of miR-346 in esophageal carcinoma and cell lines was significantly decreased(P<0.01),TE-1 cells decreased most significantly(P<0.01).The cell viability[(0.93±0.06)vs.(1.37±0.06)]and proliferation ability[(232.3±34.40)±(82.55±9.66)]of cells infected with miR-346 were decreased[(8.26%±0.80%)vs.(0.94%±0.28%),P<0.01],the apoptosis rate was increased(P<0.01).The expression of YAP1,Cyclin E and cIAP1 protein was also significantly decreased(P<0.01).Conclusion miR-346 is low-expressed in esophageal cancer tissues and cells,which can significantly inhibit the proliferation and induce apoptosis of TE-1 cells.The mechanism may be that the expression of YAP1 gene is interfered by miR-346.

关 键 词：微RNAS 食管肿瘤 Yes相关蛋白1 增殖 凋亡 

分 类 号：R735.1[医药卫生—肿瘤]